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Highly Sensitive Hy...
Highly Sensitive Hydrogen Peroxide Biosensor Based on Tobacco Peroxidase Immobilized on p-Phenylenediamine Diazonium Cation Grafted Carbon Nanotubes : Preventing Fenton-like Inactivation at Negative Potential
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- Ciogli, Leonardo (author)
- Sapienza University of Rome
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- Zumpano, Rosaceleste (author)
- Sapienza University of Rome
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- Poloznikov, Andrey A. (author)
- National Research University Higher School of Economics, Moscow
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- Hushpulian, Dmitry M. (author)
- National Research University Higher School of Economics, Moscow
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- Tishkov, Vladimir I. (author)
- Sternberg Astronomical Institute
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- Andreu, Rafael (author)
- University of Seville
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- Gorton, Lo (author)
- Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Mazzei, Franco (author)
- Sapienza University of Rome
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- Favero, Gabriele (author)
- Sapienza University of Rome
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- Bollella, Paolo (author)
- Clarkson University,University of Bari Aldo Moro
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(creator_code:org_t)
- 2021-07
- 2021
- English 10 s.
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In: ChemElectroChem. - : Wiley. - 2196-0216. ; 8:13, s. 2495-2504
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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Abstract
Subject headings
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- Herein, we present a novel electrode platform for H2O2 detection based on the immobilization of recombinant Tobacco Peroxidase (r-TOP) onto graphite electrodes (G) modified with p-phenylenediamine (p-PD) diazonium cation grafted multi-walled carbon nanotubes (MWCNTs). The employment of both p-phenylenediamine moieties and covalent cross-linking by using glutaraldehyde allowed us to enhance the sensitivity, stability, and selectivity toward H2O2 detection, as well as preventing enzyme inactivation due to the electro-Fenton reaction. This reaction continuously produces hydroxyl radicals, whose high and unselective reactivity is likely to reduce drastically the operating life of the biosensor. The protection against the electro-Fenton reaction is mainly ascribed to a beneficial enzyme immobilization leading to a correct orientation achieved through cross-linking the enzyme in combination with interaction between the uncoupled -NH2 groups (mainly uncharged at pH 7, considering a pKa of 4.6) available on the electrode surface and the enzyme. In particular, the electrode based on the r-TOP/p-PD/MWCNTs/G platform showed a lower limit of detection of 1.8 μM H2O2, an extended linear range between 6 and 900 μM H2O2, as well as a significant increase in sensitivity (63.1±0.1 μA mM−1 cm−2) compared with previous work based on TOP. Finally, the r-TOP/p-PD/MWCNTs/G electrode was tested in several H2O2 spiked food samples as a screening analytical method for the detection of H2O2.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- electro-Fenton reaction
- hydrogen peroxide biosensor
- modified electrodes
- p-phenylenediamine (p-PD) diazonium cations
- recombinant Tobacco Peroxidase (r-TOP)
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Ciogli, Leonardo
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Zumpano, Rosacel ...
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Poloznikov, Andr ...
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Hushpulian, Dmit ...
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Tishkov, Vladimi ...
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Andreu, Rafael
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show more...
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Gorton, Lo
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Mazzei, Franco
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Favero, Gabriele
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Bollella, Paolo
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
- Articles in the publication
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ChemElectroChem
- By the university
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Lund University