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  • Rörby, EmmaLund University,Lunds universitet,Avdelningen för molekylärmedicin och genterapi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Molecular Medicine and Gene Therapy,Department of Laboratory Medicine,Faculty of Medicine,Skåne University Hospital,Lund University Hospital, Sweden (author)

The stem cell regulator PEDF is dispensable for maintenance and function of hematopoietic stem cells

  • Article/chapterEnglish2017

Publisher, publication year, extent ...

  • 2017-08-31
  • Springer Science and Business Media LLC,2017

Numbers

  • LIBRIS-ID:oai:lup.lub.lu.se:6b0c2cb7-f361-44fb-8060-fb5338b6af3e
  • https://lup.lub.lu.se/record/6b0c2cb7-f361-44fb-8060-fb5338b6af3eURI
  • https://doi.org/10.1038/s41598-017-09452-2DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-140960URI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • Funding Agencies|Hemato-Linne grant (Swedish Research Council Linnaeus); STEMTHERAPY (Swedish Research Council); Swedish Cancer Society (Cancerfonden); Swedish Childhood Cancer Foundation; Swedish Research Council; Royal Swedish Academy of Sciences - Tobias Foundation; Lund University Hospital (ALF grant); Royal Physiographic Society in Lund (Kungliga Fysiograftska sallskapet); center of excellence grant in Life Sciences from the Swedish Foundation for Strategic Research
  • Pigment epithelium derived factor (PEDF), a ubiquitously expressed 50 kDa secreted glycoprotein, was recently discovered to regulate self-renewal of neural stem cells and have a supportive effect on human embryonic stem cell growth. Here, we analyzed expression of PEDF in the murine hematopoietic stem cell (HSC) compartments and found that PEDF is highly expressed in primary long-term HSCs. Therefore, we characterized the hematopoietic system in a knockout mouse model for PEDF and using this model we surprisingly found that PEDF is dispensable for HSC regulation. PEDF knockout mice exhibit normal hematopoiesis in steady state conditions and the absence of PEDF lead to normal regeneration capacity in a serial competitive transplantation setting. Additionally, PEDF-deficient cells exhibit unaltered lineage distribution upon serial transplantations. When human cord blood stem and progenitor cells were cultured in media supplemented with recombinant PEDF they did not show changes in growth potential. Taken together, we report that PEDF is not a critical regulatory factor for HSC function during regeneration in vivo or growth of human stem/progenitor cells in vitro.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Billing, MatildaLund University,Lunds universitet,Avdelningen för molekylärmedicin och genterapi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Molecular Medicine and Gene Therapy,Department of Laboratory Medicine,Faculty of Medicine,Skåne University Hospital,Lund University Hospital, Sweden(Swepub:lu)med-mn_ (author)
  • Dahl, MariaLund University,Lunds universitet,Avdelningen för molekylärmedicin och genterapi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Hematopoes och genterapi,Forskargrupper vid Lunds universitet,Division of Molecular Medicine and Gene Therapy,Department of Laboratory Medicine,Faculty of Medicine,Hematopoiesis and Gene Therapy,Lund University Research Groups,Skåne University Hospital,Lund University Hospital, Sweden(Swepub:lu)med-mrd (author)
  • Warsi, SarahLund University,Lunds universitet,Avdelningen för molekylärmedicin och genterapi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Hematopoes och genterapi,Forskargrupper vid Lunds universitet,Division of Molecular Medicine and Gene Therapy,Department of Laboratory Medicine,Faculty of Medicine,Hematopoiesis and Gene Therapy,Lund University Research Groups,Skåne University Hospital,Lund University Hospital, Sweden(Swepub:lu)med-swr (author)
  • Andradottir, SiljaLund University,Lunds universitet,Avdelningen för molekylärmedicin och genterapi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Molecular Medicine and Gene Therapy,Department of Laboratory Medicine,Faculty of Medicine,Skåne University Hospital,Lund University Hospital, Sweden(Swepub:lu)med-sla (author)
  • Miharada, KenichiLund University,Lunds universitet,Avdelningen för molekylärmedicin och genterapi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stamcellsmetabolism,Forskargrupper vid Lunds universitet,Division of Molecular Medicine and Gene Therapy,Department of Laboratory Medicine,Faculty of Medicine,Stem Cell Metabolism,Lund University Research Groups,Skåne University Hospital,Lund University Hospital, Sweden(Swepub:lu)med-kmh (author)
  • Siva, KavithaLund University,Lunds universitet,Avdelningen för molekylärmedicin och genterapi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Molecular Medicine and Gene Therapy,Department of Laboratory Medicine,Faculty of Medicine,Skåne University Hospital,Lund University Hospital, Sweden(Swepub:lu)med-kvs (author)
  • Jönsson, Jan-IngvarLinköpings universitet,Linköping University,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten(Swepub:liu)janjo86 (author)
  • Blank, UlrikaSkåne University Hospital,Lund University Hospital, Sweden (author)
  • Karlsson, GöranLund University,Lunds universitet,Avdelningen för molekylär hematologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cells and Leukemia,Forskargrupper vid Lunds universitet,Division of Molecular Hematology (DMH),Department of Laboratory Medicine,Faculty of Medicine,Lund University Research Groups,Lund University, Sweden(Swepub:lu)molm-gka (author)
  • Karlsson, StefanLund University,Lunds universitet,Avdelningen för molekylärmedicin och genterapi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Hematopoes och genterapi,Forskargrupper vid Lunds universitet,Division of Molecular Medicine and Gene Therapy,Department of Laboratory Medicine,Faculty of Medicine,Hematopoiesis and Gene Therapy,Lund University Research Groups,Lund University Hospital, Sweden(Swepub:lu)molm-ska (author)
  • Avdelningen för molekylärmedicin och genterapiInstitutionen för laboratoriemedicin (creator_code:org_t)

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  • In:Scientific Reports: Springer Science and Business Media LLC72045-2322

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