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  • Pigeot, SébastienUniversity Hospital Basel (författare)

Orthotopic Bone Formation by Streamlined Engineering and Devitalization of Human Hypertrophic Cartilage

  • Artikel/kapitelEngelska2020

Förlag, utgivningsår, omfång ...

  • 2020-09-30
  • MDPI AG,2020

Nummerbeteckningar

  • LIBRIS-ID:oai:lup.lub.lu.se:6b8034c4-6268-447d-bd2a-f6e5e313ae29
  • https://lup.lub.lu.se/record/6b8034c4-6268-447d-bd2a-f6e5e313ae29URI
  • https://doi.org/10.3390/ijms21197233DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:art swepub-publicationtype
  • Ämneskategori:ref swepub-contenttype

Anmärkningar

  • Most bones of the human body form and heal through endochondral ossification, whereby hypertrophic cartilage (HyC) is formed and subsequently remodeled into bone. We previously demonstrated that HyC can be engineered from human mesenchymal stromal cells (hMSC), and subsequently devitalized by apoptosis induction. The resulting extracellular matrix (ECM) tissue retained osteoinductive properties, leading to ectopic bone formation. In this study, we aimed at engineering and devitalizing upscaled quantities of HyC ECM within a perfusion bioreactor, followed by in vivo assessment in an orthotopic bone repair model. We hypothesized that the devitalized HyC ECM would outperform a clinical product currently used for bone reconstructive surgery. Human MSC were genetically engineered with a gene cassette enabling apoptosis induction upon addition of an adjuvant. Engineered hMSC were seeded, differentiated, and devitalized within a perfusion bioreactor. The resulting HyC ECM was subsequently implanted in a 10-mm rabbit calvarial defect model, with processed human bone (Maxgraft®) as control. Human MSC cultured in the perfusion bioreactor generated a homogenous HyC ECM and were efficiently induced towards apoptosis. Following six weeks of in vivo implantation, microcomputed tomography and histological analyses of the defects revealed an increased bone formation in the defects filled with HyC ECM as compared to Maxgraft®. This work demonstrates the suitability of engineered devitalized HyC ECM as a bone substitute material, with a performance superior to a state-of-the-art commercial graft. Streamlined generation of the devitalized tissue transplant within a perfusion bioreactor is relevant towards standardized and automated manufacturing of a clinical product.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Bourgine, Paul EmileLund University,Lunds universitet,Molekylär skelettbiologi,Forskargrupper vid Lunds universitet,WCMM- Wallenberg center för molekylär medicinsk forskning,Medicinska fakulteten,Molecular Skeletal Biology,Lund University Research Groups,WCMM-Wallenberg Centre for Molecular Medicine,Faculty of Medicine,University Hospital Basel(Swepub:lu)pa2431bo (författare)
  • Claude, JaquieryUniversity Hospital Basel (författare)
  • Scotti, CelesteIstituto Ortopedico Galeazzi,Novartis Institutes for BioMedical Research, Inc. (författare)
  • Papadimitropoulos, AdamUniversity Hospital Basel (författare)
  • Todorov, AtanasUniversity Hospital Basel (författare)
  • Epple, ChristianUniversity Hospital Basel (författare)
  • Peretti, Giuseppe MUniversity of Milan,Istituto Ortopedico Galeazzi (författare)
  • Martin, IvanUniversity Hospital Basel (författare)
  • University Hospital BaselMolekylär skelettbiologi (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:International Journal of Molecular Sciences: MDPI AG21:191422-0067

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