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Label-free acoustop...
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Urbansky, AnkeLund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Acoustofluidics group,Forskargrupper vid Lunds universitet,Other operations, LTH,Faculty of Engineering, LTH,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Lund University Research Groups
(author)
Label-free acoustophoretic enrichment of mononuclear cells from blood
- Article/chapterEnglish2017
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LIBRIS-ID:oai:lup.lub.lu.se:6c4f34bc-4543-4cdd-93bd-41b774616e5a
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https://lup.lub.lu.se/record/6c4f34bc-4543-4cdd-93bd-41b774616e5aURI
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Language:English
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Summary in:English
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Subject category:kon swepub-publicationtype
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Subject category:ref swepub-contenttype
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We are reporting an efficient, label-free and continuous separation of mononuclear cells (MNC), from blood using microchip acoustophoresis. In standard PBS buffer, MNCs and red blood cells (RBC) display overlapping acoustophoretic mobilities which compromise separation of these cell types from each other. In this paper we capitalize on the fact that MNC and RBC display different acoustophysical properties. By optimizing the buffer conditions and thereby changing the acoustic contrast factor, and hence the acoustophoretic mobility of the cells, a 2800-fold enrichment of MNCs vs. RBCs with MNC recoveries up to 88% was accomplished.
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Ohlsson, PelleLund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Acoustofluidics group,Forskargrupper vid Lunds universitet,Other operations, LTH,Faculty of Engineering, LTH,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Lund University Research Groups,AcouSort AB(Swepub:lu)elma-poo
(author)
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Lenshof, AndreasLund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Acoustofluidics group,Forskargrupper vid Lunds universitet,Other operations, LTH,Faculty of Engineering, LTH,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Lund University Research Groups(Swepub:lu)elma-ani
(author)
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Scheding, StefanLund University,Lunds universitet,Benmärgsstamceller och cellulära terapier,Forskargrupper vid Lunds universitet,Bone marrow stem cells and cellular therapies,Lund University Research Groups,Skåne University Hospital(Swepub:lu)stem-ssc
(author)
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Laurell, ThomasLund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Acoustofluidics group,Forskargrupper vid Lunds universitet,Other operations, LTH,Faculty of Engineering, LTH,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,Lund University Research Groups,Dongguk University, Seoul(Swepub:lu)elma-tla
(author)
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NanoLund: Centre for NanoscienceAnnan verksamhet, LTH
(creator_code:org_t)
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In:MicroTAS 2017 : Savannah, Georgia, USA - Savannah, Georgia, USA2017, s. 1309-13101556-59049780692941836
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