Hyphal exploration strategies and habitat modification of an arbuscular mycorrhizal fungus in microengineered soil chips

Hammer, Edith C.Lund University,Lunds universitet,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science (författare)

Arbuscular mycorrhizal fungi (AMF) are considered ecosystem engineers, but the interactions of their mycelium with their immediate surroundings are largely unknown. In this study, we used microfluidic chips, simulating artificial soil structures, to study foraging strategies and habitat modification of Rhizophagus irregularis symbiotically associated to carrot roots. AMF hyphae foraged over long distances in nutrient-void spaces, preferred straight over tortuous passages, anastomosed and showed strong inducement of branching when encountering obstacles. We measured bi-directional transport of cellular content inside active hyphae and documented strategic allocation of biomass within the mycelium via cytoplasm retraction from inefficient paths. R. irregularis modified pore-spaces in the chips by clogging pores with irregularly shaped spores. We suggest that studying AMF hyphal behaviour in spatial settings can explain phenomena reported at bulk scale such as AMF modification of water retention in soils. The use of microfluidic soil chips in AMF research opens up novel opportunities to study their ecophysiology and interactions with both biotic and abiotic factors.

LANTBRUKSVETENSKAPER Lantbruksvetenskap, skogsbruk och fiske Markvetenskap hsv//swe
AGRICULTURAL SCIENCES Agriculture, Forestry and Fisheries Soil Science hsv//eng
AMF
Arbuscular mycorrhizal fungi
Behaviour
Fungi-on-a-chip
Hyphal behaviour
Microfluidics
Mycorrhizal traits
Rhizophagus irregularis
Soil chips
Soil pore space

Arellano-Caicedo, CarlosLund University (författare)
Mafla-Endara, Paola MicaelaLund University,Lunds universitet,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science(Swepub:lu)pa1613ma (författare)
Kiers, E. TobyVrije Universiteit Amsterdam (författare)
Shimizu, TomFOM Institute for Atomic and Molecular Physics (författare)
Ohlsson, PelleLund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)elma-poo (författare)
Aleklett, KristinLund University,Lunds universitet,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science(Swepub:lu)kr1346al (författare)
Biologiska institutionenNaturvetenskapliga fakulteten (creator_code:org_t)

Av författaren/redakt...: Hammer, Edith C.; Arellano-Caicedo ...; Mafla-Endara, Pa ...; Kiers, E. Toby; Shimizu, Tom; Ohlsson, Pelle; visa fler...; Aleklett, Kristi ...; visa färre...

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