In vitro evolution of a fungal laccase in high concentrations of organic cosolvents

Fungal laccases are remarkable green catalysts that have a broad substrate specificity and many potential applications in bioremediation, lignocellulose processing, organic synthesis, and more. However, most of these transformations must be carried out at high concentrations of organic cosolvents in which laccases undergo unfolding, thereby losing their activity. We have tailored a thermostable laccase that tolerates high concentrations of cosolvents, the genetic product of five rounds of directed evolution expressed in Saccharomyces cerevisiae. This evolved laccase-R2 variant-was capable of resisting a wide array of cosolvents at concentrations as high as 50% (v/v). Intrinsic laccase features such as the redox potential and the geometry of catalytic coppers varied slightly during the course of the molecular evolution. Some mutations at the protein surface stabilized the laccase by allowing additional electrostatic and hydrogen bonding to occur.

Bulter, Thomas (author)
Shleev, SergeyLund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)anal-ssh (author)
Polaina, Julio (author)
Martinez-Arias, Arturo (author)
Plow, Francisco J. (author)
Ballesteros, Antonio (author)
Alcalde, Miguel (author)
Centrum för analys och syntesKemiska institutionen (creator_code:org_t)

By the author/editor: Zumarraga, Miren; Bulter, Thomas; Shleev, Sergey; Polaina, Julio; Martinez-Arias, ...; Plow, Francisco ...; show more...; Ballesteros, Ant ...; Alcalde, Miguel; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Analytical Chemi ...

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