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A Specific Calprote...
A Specific Calprotectin Neo-epitope [CPa9-HNE] in Serum from Inflammatory Bowel Disease Patients Is Associated with Neutrophil Activity and Endoscopic Severity
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- Mortensen, Joachim Høg (författare)
- Nordic Bioscience AS
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- Sinkeviciute, Dovile (författare)
- Lund University,Lunds universitet,Molekylär skelettbiologi,Forskargrupper vid Lunds universitet,Molecular Skeletal Biology,Lund University Research Groups,Nordic Bioscience AS
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- Manon-Jensen, Tina (författare)
- Nordic Bioscience AS
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Domislović, Viktor (författare)
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- McCall, Kathryn (författare)
- Glasgow Royal Infirmary
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- Thudium, Christian S. (författare)
- Nordic Bioscience AS
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Brinar, Marko (författare)
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- Önnerfjord, Patrik (författare)
- Lund University,Lunds universitet,Molekylär skelettbiologi,Forskargrupper vid Lunds universitet,Molecular Skeletal Biology,Lund University Research Groups
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- Goodyear, Carl S. (författare)
- Glasgow Royal Infirmary
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Krznarić, Željko (författare)
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- Karsdal, Morten Asser (författare)
- Nordic Bioscience AS
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- Bay-Jensen, Anne Christine (författare)
- Nordic Bioscience AS
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(creator_code:org_t)
- 2022-03-19
- 2022
- Engelska 14 s.
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Ingår i: Journal of Crohn's & Colitis. - : Oxford University Press (OUP). - 1873-9946 .- 1876-4479. ; 16:9, s. 1447-1460
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- BACKGROUND AND AIMS: Endoscopy and the use of faecal calprotectin [faecal CP] are among the least-favoured methods for assessing disease activity by inflammatory bowel disease [IBD] patients; the handling/processing of faecal samples is also impractical. Therefore, we sought to develop a novel neo-epitope serum calprotectin enzyme-linked immunosorbent assay [ELISA], CPa9-HNE, with the aim of quantifying neutrophil activity and neutrophil extracellular trap [NET]-osis and proposing a non-invasive method for monitoring disease activity in IBD patients. METHODS: In vitro cleavage was performed by mixing calprotectin [S100A9/S100A8] with human neutrophil elastase [HNE], and a novel HNE-derived calprotectin neo-epitope [CPa9-HNE] was identified by mass spectrometry for ELISA development. The CPa9-HNE ELISA was quantified in supernatants from ex vivo activated neutrophils and serum samples from patients with ulcerative colitis [UC, n = 43], Crohn's disease [CD, n = 93], and healthy subjects [HS, n = 23]. For comparison, faecal CP and MRP8/14 biomarkers were also measured. RESULTS: CPa9-HNE was specific for activated neutrophils ex vivo. Serum CPa9-HNE levels were 4-fold higher in CD [p <0.0001] and UC [p <0.0001] patients than in HS. CPa9-HNE correlated well with the Simple Endoscopic Score [SES]-CD score [r = 0.61, p <0.0001], MES [r = 0.46, p = 0.0141], and the full Mayo score [r = 0.52, p = 0.0013]. CPa9-HNE was able to differentiate between CD and UC patients in endoscopic remission and moderate/severe disease activity (CD: area under the curve [AUC] = 0.82 [p = 0.0003], UC: AUC = 0.87 [p = 0.0004]). The performance of CPa9-HNE was equipotent or slightly better than that of faecal CP. CONCLUSIONS: Serum CPa9-HNE levels were highly associated with CD and UC patients. CPa9-HNE correlated with the SES-CD score and the full Mayo score, indicating a strong association with disease activity.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Gastroenterologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)
Nyckelord
- biomarkers
- Calprotectin
- IBD
- inflammation
- neutrophil elastase
- neutrophil extracellular traps [NETs]
- neutrophil granulocyte
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Mortensen, Joach ...
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Sinkeviciute, Do ...
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Manon-Jensen, Ti ...
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Domislović, Vikt ...
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McCall, Kathryn
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Thudium, Christi ...
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visa fler...
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Brinar, Marko
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Önnerfjord, Patr ...
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Goodyear, Carl S ...
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Krznarić, Željko
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Karsdal, Morten ...
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Bay-Jensen, Anne ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Gastroenterologi
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Journal of Crohn ...
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Lunds universitet