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A Specific Calprote...
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Mortensen, Joachim HøgNordic Bioscience AS
(författare)
A Specific Calprotectin Neo-epitope [CPa9-HNE] in Serum from Inflammatory Bowel Disease Patients Is Associated with Neutrophil Activity and Endoscopic Severity
- Artikel/kapitelEngelska2022
Förlag, utgivningsår, omfång ...
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2022-03-19
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Oxford University Press (OUP),2022
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14 s.
Nummerbeteckningar
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LIBRIS-ID:oai:lup.lub.lu.se:731d5a69-e188-4460-811c-0f3b57a1efc7
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https://lup.lub.lu.se/record/731d5a69-e188-4460-811c-0f3b57a1efc7URI
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https://doi.org/10.1093/ecco-jcc/jjac047DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
Anmärkningar
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BACKGROUND AND AIMS: Endoscopy and the use of faecal calprotectin [faecal CP] are among the least-favoured methods for assessing disease activity by inflammatory bowel disease [IBD] patients; the handling/processing of faecal samples is also impractical. Therefore, we sought to develop a novel neo-epitope serum calprotectin enzyme-linked immunosorbent assay [ELISA], CPa9-HNE, with the aim of quantifying neutrophil activity and neutrophil extracellular trap [NET]-osis and proposing a non-invasive method for monitoring disease activity in IBD patients. METHODS: In vitro cleavage was performed by mixing calprotectin [S100A9/S100A8] with human neutrophil elastase [HNE], and a novel HNE-derived calprotectin neo-epitope [CPa9-HNE] was identified by mass spectrometry for ELISA development. The CPa9-HNE ELISA was quantified in supernatants from ex vivo activated neutrophils and serum samples from patients with ulcerative colitis [UC, n = 43], Crohn's disease [CD, n = 93], and healthy subjects [HS, n = 23]. For comparison, faecal CP and MRP8/14 biomarkers were also measured. RESULTS: CPa9-HNE was specific for activated neutrophils ex vivo. Serum CPa9-HNE levels were 4-fold higher in CD [p <0.0001] and UC [p <0.0001] patients than in HS. CPa9-HNE correlated well with the Simple Endoscopic Score [SES]-CD score [r = 0.61, p <0.0001], MES [r = 0.46, p = 0.0141], and the full Mayo score [r = 0.52, p = 0.0013]. CPa9-HNE was able to differentiate between CD and UC patients in endoscopic remission and moderate/severe disease activity (CD: area under the curve [AUC] = 0.82 [p = 0.0003], UC: AUC = 0.87 [p = 0.0004]). The performance of CPa9-HNE was equipotent or slightly better than that of faecal CP. CONCLUSIONS: Serum CPa9-HNE levels were highly associated with CD and UC patients. CPa9-HNE correlated with the SES-CD score and the full Mayo score, indicating a strong association with disease activity.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Sinkeviciute, DovileLund University,Lunds universitet,Molekylär skelettbiologi,Forskargrupper vid Lunds universitet,Molecular Skeletal Biology,Lund University Research Groups,Nordic Bioscience AS(Swepub:lu)do6165si
(författare)
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Manon-Jensen, TinaNordic Bioscience AS
(författare)
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Domislović, Viktor
(författare)
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McCall, KathrynGlasgow Royal Infirmary
(författare)
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Thudium, Christian S.Nordic Bioscience AS(Swepub:lu)med-ctu
(författare)
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Brinar, Marko
(författare)
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Önnerfjord, PatrikLund University,Lunds universitet,Molekylär skelettbiologi,Forskargrupper vid Lunds universitet,Molecular Skeletal Biology,Lund University Research Groups(Swepub:lu)akem-pon
(författare)
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Goodyear, Carl S.Glasgow Royal Infirmary
(författare)
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Krznarić, Željko
(författare)
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Karsdal, Morten AsserNordic Bioscience AS
(författare)
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Bay-Jensen, Anne ChristineNordic Bioscience AS
(författare)
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Nordic Bioscience ASMolekylär skelettbiologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Crohn's & Colitis: Oxford University Press (OUP)16:9, s. 1447-14601873-99461876-4479
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Mortensen, Joach ...
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Sinkeviciute, Do ...
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Manon-Jensen, Ti ...
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Domislović, Vikt ...
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McCall, Kathryn
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Thudium, Christi ...
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Brinar, Marko
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Önnerfjord, Patr ...
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Goodyear, Carl S ...
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Krznarić, Željko
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Karsdal, Morten ...
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Bay-Jensen, Anne ...
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och Klinisk medicin
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Lunds universitet