Enterococcus faecalis Infection Causes Inflammation, Intracellular Oxphos-Independent ROS Production, and DNA Damage in Human Gastric Cancer Cells

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: WFRF:(Juel Rasmussen Lene) > (2013) > Enterococcus faecal...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Strickertsson, Jesper A. B. (author)

Enterococcus faecalis Infection Causes Inflammation, Intracellular Oxphos-Independent ROS Production, and DNA Damage in Human Gastric Cancer Cells

Article/chapterEnglish2013

Publisher, publication year, extent ...

2013-04-30
Public Library of Science (PLoS),2013
electronicrdacarrier

Numbers

LIBRIS-ID:oai:lup.lub.lu.se:7611917a-31f9-4e2e-bf16-26db095c3726
https://lup.lub.lu.se/record/3931227URI
https://doi.org/10.1371/journal.pone.0063147DOI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:art swepub-publicationtype
Subject category:ref swepub-contenttype

Notes

Background: Achlorhydria caused by e.g. atrophic gastritis allows for bacterial overgrowth, which induces chronic inflammation and damage to the mucosal cells of infected individuals driving gastric malignancies and cancer. Enterococcus faecalis (E. faecalis) can colonize achlohydric stomachs and we therefore wanted to study the impact of E. faecalis infection on inflammatory response, reactive oxygen species (ROS) formation, mitochondrial respiration, and mitochondrial genetic stability in gastric mucosal cells. Methods: To separate the changes induced by bacteria from those of the inflammatory cells we established an in vitro E. faecalis infection model system using the gastric carcinoma cell line MKN74. Total ROS and superoxide was measured by fluorescence microscopy. Cellular oxygen consumption was characterized non-invasively using XF24 microplate based respirometry. Gene expression was examined by microarray, and response pathways were identified by Gene Set Analysis (GSA). Selected gene transcripts were verified by quantitative real-time polymerase chain reaction (qRT-PCR). Mitochondrial mutations were determined by sequencing. Results: Infection of MKN74 cells with E. faecalis induced intracellular ROS production through a pathway independent of oxidative phosphorylation (oxphos). Furthermore, E. faecalis infection induced mitochondrial DNA instability. Following infection, genes coding for inflammatory response proteins were transcriptionally up-regulated while DNA damage repair and cell cycle control genes were down-regulated. Cell growth slowed down when infected with viable E. faecalis and responded in a dose dependent manner to E. faecalis lysate. Conclusions: Infection by E. faecalis induced an oxphos-independent intracellular ROS response and damaged the mitochondrial genome in gastric cell culture. Finally the bacteria induced an NF-kappa B inflammatory response as well as impaired DNA damage response and cell cycle control gene expression.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

Desler, Claus (author)
Martin-Bertelsen, TomasUniversity of Copenhagen(Swepub:lu)thep-tme (author)
Machado, Ana Manuel Dantas (author)
Wadström, TorkelLund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)mmb-twa (author)
Winther, Ole (author)
Rasmussen, Lene Juel (author)
Friis-Hansen, Lennart (author)
University of CopenhagenAvdelningen för medicinsk mikrobiologi (creator_code:org_t)

Related titles

In:PLoS ONE: Public Library of Science (PLoS)8:41932-6203

Internet link

Find in a library

PLoS ONE (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Strickertsson, J ...; Desler, Claus; Martin-Bertelsen ...; Machado, Ana Man ...; Wadström, Torkel; Winther, Ole; show more...; Rasmussen, Lene ...; Friis-Hansen, Le ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Microbiology in ...

Articles in the publication: PLoS ONE

By the university: Lund University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se