The immune diversity in a test tube - Non-immunised antibody libraries and functional variability in defined protein scaffolds

• Technologies to develop and evolve the function of proteins and, in particular, antibodies have developed rapidly since the introduction of phage display. Importantly, it has become possible to identify molecules with binding properties that cannot be found by other means. A range of different approaches to create general libraries that are useful for the selection of such molecules specific for essentially any kind of target have emerged. We herein review some of the most prominent approaches in the field and in particular discuss specific features related to the development of antibody libraries based on single antibody framework scaffolds. This approach not only permits identification of a range of specific binders, but also facilitates further evolution of initially derived molecules into molecules with optimised functions.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Immunology in the medical area (hsv//eng)

Nyckelord

COMPLEMENTARITY-DETERMINING REGIONS

PHAGE DISPLAY LIBRARY

SINGLE-CHAIN

FV

AFFINITY HUMAN-ANTIBODIES

BY-PASSING IMMUNIZATION

RANDOM

MUTAGENESIS

BINDING-SITE

V-H

SYNTHETIC REPERTOIRES

SOMATIC

HYPERMUTATION

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