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Analytical validati...
Analytical validation of a standardized scoring protocol for Ki67 immunohistochemistry on breast cancer excision whole sections: an international multicenter collaboration
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- Leung, Samuel CY (författare)
- University of British Columbia
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- Nielsen, Torsten (författare)
- University of British Columbia
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- Zabaglo, Lila A (författare)
- Institute of Cancer Research London
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- Arun, Indu (författare)
- Tata Medical Centre, Kolkata
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- Badve, Sunil S (författare)
- Indiana University
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- Bane, Anita L (författare)
- McMaster University
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- Bartlett, John (författare)
- Ontario Institute for Cancer Research,Western General Hospital
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- Borgquist, Signe (författare)
- Lund University,Lunds universitet,Bröstcancer - prevention & intervention,Forskargrupper vid Lunds universitet,Breast cancer prevention & intervention,Lund University Research Groups
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- Chang, Martin C (författare)
- University of Vermont
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- Dodson, A (författare)
- Royal Marsden Hospital, London
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- Ehinger, Anna (författare)
- Lund University,Lunds universitet,Individuell Bröstcancerbehandling,Forskargrupper vid Lunds universitet,Personalized Breast Cancer Treatment,Lund University Research Groups,Skåne University Hospital
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- Fineberg, Susan (författare)
- University of Vermont Medical Center (UVM)
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- Focke, Cornelia (författare)
- Dietrich-Bonhoeffer-Klinikum
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- Gao, Dongxia (författare)
- University of British Columbia
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- Gown, Allen M (författare)
- PhenoPath Laboratories
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- Gutierrez, Carolina (författare)
- Baylor College of Medicine
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- Hugh, Judith C (författare)
- University of Alberta
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- Kos, Zuzana (författare)
- The Ottawa Hospital,University of Ottawa
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- Laenkholm, Anne Vibeke (författare)
- University College Zealand,Slagelse Hospital
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- Mastropasqua, Mauro G (författare)
- European Institute of Oncology
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- Moriya, Takuya (författare)
- Kawasaki Medical School
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- Nofech-Mozes, Sharon (författare)
- University of Toronto,Sunnybrook Health Sciences Centre
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- Osborne, C Kent (författare)
- Baylor College of Medicine
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- Penault-Llorca, Frédérique M (författare)
- University of Auvergne
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- Piper, Tammy (författare)
- Western General Hospital
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- Sakatani, Takashi (författare)
- Nippon Medical School
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- Salgado, Roberto (författare)
- University of Antwerp,Peter MacCallum Cancer Centre
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- Starczynski, Jane (författare)
- Birmingham Heartlands Hospital
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- Sugie, Tomoharu (författare)
- Kansai Medical University
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- van der Vegt, Bert (författare)
- University Medical Center Groningen
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- Viale, Giuseppe (författare)
- University of Milan,European Institute of Oncology
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- Hayes, Daniel (författare)
- University of Michigan
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- McShane, Lisa M (författare)
- National Cancer Institute, NCI
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- Dowsett, Mitch (författare)
- Institute of Cancer Research London
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(creator_code:org_t)
- 2019-07-08
- 2019
- Engelska 11 s.
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Ingår i: Histopathology. - : Wiley. - 0309-0167 .- 1365-2559. ; 75:2, s. 225-235
- Relaterad länk:
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http://dx.doi.org/10...
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http://deepblue.lib....
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Aims: The nuclear proliferation marker Ki67 assayed by immunohistochemistry has multiple potential uses in breast cancer, but an unacceptable level of interlaboratory variability has hampered its clinical utility. The International Ki67 in Breast Cancer Working Group has undertaken a systematic programme to determine whether Ki67 measurement can be analytically validated and standardised among laboratories. This study addresses whether acceptable scoring reproducibility can be achieved on excision whole sections.Methods and results: Adjacent sections from 30 primary ER+ breast cancers were centrally stained for Ki67 and sections were circulated among 23 pathologists in 12 countries. All pathologists scored Ki67 by two methods: (i) global: four fields of 100 tumour cells each were selected to reflect observed heterogeneity in nuclear staining; (ii) hot‐spot: the field with highest apparent Ki67 index was selected and up to 500 cells scored. The intraclass correlation coefficient (ICC) for the global method [confidence interval (CI) = 0.87; 95% CI = 0.799–0.93] marginally met the prespecified success criterion (lower 95% CI ≥ 0.8), while the ICC for the hot‐spot method (0.83; 95% CI = 0.74–0.90) did not. Visually, interobserver concordance in location of selected hot‐spots varies between cases. The median times for scoring were 9 and 6 min for global and hot‐spot methods, respectively.Conclusions: The global scoring method demonstrates adequate reproducibility to warrant next steps towards evaluation for technical and clinical validity in appropriate cohorts of cases. The time taken for scoring by either method is practical using counting software we are making publicly available. Establishment of external quality assessment schemes is likely to improve the reproducibility between laboratories further.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Leung, Samuel CY
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Nielsen, Torsten
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Zabaglo, Lila A
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Arun, Indu
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Badve, Sunil S
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Bane, Anita L
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visa fler...
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Bartlett, John
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Borgquist, Signe
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Chang, Martin C
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Dodson, A
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Ehinger, Anna
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Fineberg, Susan
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Focke, Cornelia
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Gao, Dongxia
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Gown, Allen M
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Gutierrez, Carol ...
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Hugh, Judith C
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Kos, Zuzana
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Laenkholm, Anne ...
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Mastropasqua, Ma ...
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Moriya, Takuya
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Nofech-Mozes, Sh ...
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Osborne, C Kent
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Penault-Llorca, ...
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Piper, Tammy
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Sakatani, Takash ...
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Salgado, Roberto
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Starczynski, Jan ...
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Sugie, Tomoharu
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van der Vegt, Be ...
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Viale, Giuseppe
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Hayes, Daniel
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McShane, Lisa M
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Dowsett, Mitch
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Cancer och onkol ...
- Artiklar i publikationen
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Histopathology
- Av lärosätet
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Lunds universitet