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Fusion of oil bodie...
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Heneen, Waheeb K.
(author)
Fusion of oil bodies in endosperm of oat grains
- Article/chapterEnglish2008
Publisher, publication year, extent ...
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2008-06-19
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Springer Science and Business Media LLC,2008
Numbers
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LIBRIS-ID:oai:lup.lub.lu.se:85f06c75-064d-4f2a-938a-4d3c44804f8a
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https://lup.lub.lu.se/record/1247159URI
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https://doi.org/10.1007/s00425-008-0761-xDOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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Few microscopical studies have been made on lipid storage in oat grains, with variable results as to the extent of lipid accumulation in the starchy endosperm. Grains of medium- and high-lipid oat (Avena sativa L.) were studied at two developmental stages and at maturity, by light microscopy using different staining methods, and by scanning and transmission electron microscopy. Discrete oil bodies occurred in the aleurone layer, scutellum and embryo. In contrast, oil bodies in the starchy endosperm often had diffuse boundaries and fused with each other and with protein vacuoles during grain development, forming a continuous oil matrix between the protein and starch components. The different microscopical methods were confirmative to each other regarding the coalescence of oil bodies, a phenomenon probably correlated with the reduced amount of oil-body associated proteins in the endosperm. This was supported experimentally by SDS-PAGE separation of oil-body proteins and immunoblotting and immunolocalization with antibodies against a 16 kD oil-body protein. Much more oil-body proteins per amount of oil occurred in the embryo and scutellum than in the endosperm. Immunolocalization of 14 and 16 kD oil-body associated proteins on sectioned grains resulted in more heavy labeling of the embryo, scutellum and aleurone layer than the rest of the endosperm. Observations on the appearance of oil bodies at an early stage of development pertain to the prevailing hypotheses of oil-body biogenesis.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Karlsson, GunnelLund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)ook2-gka
(author)
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Brismar, Kerstin
(author)
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Gummeson, Per-Olov
(author)
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Marttila, Salla
(author)
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Leonova, Svetlana
(author)
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Carlsson, Anders S.
(author)
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Bafor, Maureen
(author)
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Banas, Antoni
(author)
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Mattsson, Bengt
(author)
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Debski, Henryk
(author)
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Stymne, Sten
(author)
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Centrum för analys och syntesKemiska institutionen
(creator_code:org_t)
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In:Planta: Springer Science and Business Media LLC228:4, s. 589-5990032-09351432-2048
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Brismar, Kerstin
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Carlsson, Anders ...
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Banas, Antoni
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Mattsson, Bengt
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Stymne, Sten
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