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The Tetraspanin CD9 Affords High-Purity Capture of All Murine Hematopoietic Stem Cells

Karlsson, Göran (author)
University College London
Rörby, Emma (author)
Lund University,Lunds universitet,Avdelningen för molekylärmedicin och genterapi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Molecular Medicine and Gene Therapy,Department of Laboratory Medicine,Faculty of Medicine
Pina, Cristina (author)
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Soneji, Shamit (author)
Lund University,Lunds universitet,Avdelningen för molekylärmedicin och genterapi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Molecular Medicine and Gene Therapy,Department of Laboratory Medicine,Faculty of Medicine
Reckzeh, Kristian (author)
Miharada, Kenichi (author)
Lund University,Lunds universitet,Avdelningen för molekylärmedicin och genterapi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stamcellsmetabolism,Forskargrupper vid Lunds universitet,Division of Molecular Medicine and Gene Therapy,Department of Laboratory Medicine,Faculty of Medicine,Stem Cell Metabolism,Lund University Research Groups
Karlsson, Christine (author)
Lund University,Lunds universitet,Avdelningen för klinisk genetik,Institutionen för laboratoriemedicin,Medicinska fakulteten,Avdelningen för molekylärmedicin och genterapi,Division of Clinical Genetics,Department of Laboratory Medicine,Faculty of Medicine,Division of Molecular Medicine and Gene Therapy
Guo, Yanping (author)
Fugazza, Cristina (author)
Gupta, Rajeev (author)
Martens, Joost H. A. (author)
Stunnenberg, Hendrik G. (author)
Karlsson, Stefan (author)
Lund University,Lunds universitet,Avdelningen för molekylärmedicin och genterapi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Molecular Medicine and Gene Therapy,Department of Laboratory Medicine,Faculty of Medicine
Enver, Tariq (author)
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 (creator_code:org_t)
Elsevier BV, 2013
2013
English.
In: Cell Reports. - : Elsevier BV. - 2211-1247. ; 4:4, s. 642-648
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Prospective isolation is critical for understanding the cellular and molecular aspects of stem cell heterogeneity. Here, we identify the cell surface antigen CD9 as a positive marker that provides a simple alternative for hematopoietic stem cell isolation at high purity. Crucially, CD9 affords the capture of all hematopoietic stem cells in murine bone marrow in the absence of contaminating populations that lack authentic stem cell function. Using CD9 as a tool to subdivide hematopoietic stem-cell-containing populations, we provide evidence for heterogeneity at the cellular, functional, and molecular levels.

Subject headings

NATURVETENSKAP  -- Biologi -- Cellbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Cell Biology (hsv//eng)

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