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Three-dimensional m...
Three-dimensional microstructure of human meniscus posterior horn in health and osteoarthritis
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- Kestilä, I. (author)
- University of Oulu
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- Folkesson, E. (author)
- Lund University,Lunds universitet,Lund OsteoArthritis Division - Clinical Epidemiology Unit,Forskargrupper vid Lunds universitet,Molekylär skelettbiologi,Lund University Research Groups,Molecular Skeletal Biology
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- Finnilä, M. A. (author)
- University of Oulu
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- Turkiewicz, A. (author)
- Lund University,Lunds universitet,Lund OsteoArthritis Division - Clinical Epidemiology Unit,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Önnerfjord, P. (author)
- Lund University,Lunds universitet,Molekylär skelettbiologi,Forskargrupper vid Lunds universitet,Molecular Skeletal Biology,Lund University Research Groups
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- Hughes, V. (author)
- Lund University,Lunds universitet,Lund OsteoArthritis Division - Clinical Epidemiology Unit,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Tjörnstrand, J. (author)
- Lund University,Lunds universitet,Forskningsgruppen för höft- och knäproteskirurgi,Forskargrupper vid Lunds universitet,Lund OsteoArthritis Division - Höftsjukdomar från vaggan till protesen,The Hip and Knee Joint Arthroplasty Research Group,Lund University Research Groups,Lund OsteoArthritis Division - Hip diseases from the cradle to the prosthesis
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- Englund, M. (author)
- Lund University,Lunds universitet,Lund OsteoArthritis Division - Clinical Epidemiology Unit,Forskargrupper vid Lunds universitet,Lund University Research Groups,Boston University
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- Saarakkala, S. (author)
- Oulu University Hospital,University of Oulu
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(creator_code:org_t)
- Elsevier BV, 2019
- 2019
- English.
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In: Osteoarthritis and Cartilage. - : Elsevier BV. - 1063-4584. ; 27:22, s. 1790-1799
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Abstract
Subject headings
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- Objective: To develop and perform ex vivo 3D imaging of meniscus posterior horn microstructure using micro-computed tomography (μCT), and to compare specimens from healthy references against end-stage osteoarthritis (OA) using conventional section-based histology and qualitative μCT. Design: We retrieved human medial and lateral menisci from 10 deceased donors without knee OA (healthy references) and medial and lateral menisci from 10 patients having total knee replacement for medial compartment OA. Meniscal posterior horns were dissected and fixed in formalin. One subsection underwent hexamethyldisilazane (HMDS) treatment and μCT imaging. Pauli's histopathological scoring was performed for 3 other subsections. The differences in histopathological scores were estimated using mixed linear regression, resulting in fixed effects estimates for within-knee comparisons and adjusted for age and body mass index for between-subjects comparisons. Results: 3D visualization with μCT qualitatively revealed similar microstructural changes in the posterior horns as conventional histology. The mean histopathological score was higher for medial menisci from OA knees vs both medial reference menisci (mean difference [95% CI], 3.9 [2.6,5.3]), and lateral menisci from OA knees (3.9 [2.9,5.0]). The scores were similar between lateral menisci from OA knees and lateral reference menisci (0.8 [−0.6,2.2]), and between medial and lateral reference menisci (0.8 [−0.3,1.9]). Conclusions: HMDS-based μCT protocol allows unique 3D visualization of meniscus microstructures. Posterior horns of medial menisci from medial compartment OA knees had higher histopathological scores than both the lateral posterior horns from the same OA knees and medial reference menisci, suggesting a strong association between meniscus degradation and unicompartmental knee OA.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Radiologi och bildbehandling (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Radiology, Nuclear Medicine and Medical Imaging (hsv//eng)
Keyword
- Histopathological score
- Meniscus
- Micro-computed tomography
- Osteoarthritis
Publication and Content Type
- art (subject category)
- ref (subject category)
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