WFRF:(Dong Lan)
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- Lu, Lu (author)
Crystal structure of tubulin folding cofactor A from Arabidopsis thaliana and its beta-tubulin binding characterization
- Article/chapterEnglish2010
Publisher, publication year, extent ...
- 2010-07-16
- Wiley,2010
- 7 s.
Numbers
- LIBRIS-ID:oai:lup.lub.lu.se:88c0406d-5a12-4137-a2af-387abdfc8bfa
- https://lup.lub.lu.se/record/88c0406d-5a12-4137-a2af-387abdfc8bfaURI
- https://doi.org/10.1016/j.febslet.2010.07.017DOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- Microtubules are composed of polymerized alpha/beta-tubulin heterodimers. Biogenesis of assembly-competent tubulin dimers is a complex multistep process that requires sequential actions of distinct molecular chaperones and cofactors. Tubulin folding cofactor A (TFCA), which captures beta-tubulin during the folding pathway, has been identified in many organisms. Here, we report the crystal structure of Arabidopsis thaliana TFC A (KIESEL, KIS), which forms a monomeric three-helix bundle. The functional binding analysis demonstrated that KIS interacts with beta-tubulin in plant. Furthermore, mutagenesis studies indicated that the alpha-helical regions of KIS participate in beta-tubulin binding. Unlike the budding yeast TFC A, the two loop regions of KIS are not required for this interaction suggesting a distinct binding mechanism of TFC A to beta-tubulin in plants.
Subject headings and genre
- Amino Acid Sequence
- Arabidopsis
- Arabidopsis Proteins
- Crystallography, X-Ray
- Genes, Plant
- Genetic Complementation Test
- Microtubule-Associated Proteins
- Models, Molecular
- Molecular Chaperones
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Plants, Genetically Modified
- Protein Binding
- Protein Folding
- Protein Interaction Domains and Motifs
- Protein Structure, Secondary
- Recombinant Proteins
- Sequence Homology, Amino Acid
- Tubulin
Added entries (persons, corporate bodies, meetings, titles ...)
- Nan, JieLund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory,Peking University(Swepub:lu)maxl-jna (author)
- Mi, Wei (author)
- Li, Lan-Fen (author)
- Wei, Chun-Hong (author)
- Su, Xiao-DongLund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)mbfys-xs (author)
- Li, Yi (author)
- Lunds universitetMAX IV-laboratoriet (creator_code:org_t)
Related titles
- In:FEBS Letters: Wiley584:16, s. 9-35331873-34680014-5793
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- FEBS Letters
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