SwePub
Sök i LIBRIS databas

  Utökad sökning

WFRF:(Hoffman Daniel)
 

Sökning: WFRF:(Hoffman Daniel) > (2020-2024) > A Single Amino Acid...

  • Holley, Concerta L.Department of Microbiology and Immunology, Emory University School of Medicinegrid.471395.d, Atlanta, Georgia, USA (författare)

A Single Amino Acid Substitution in Elongation Factor G Can Confer Low-Level Gentamicin Resistance in Neisseria gonorrhoeae

  • Artikel/kapitelEngelska2022

Förlag, utgivningsår, omfång ...

  • American Society for Microbiology,2022
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:oru-98802
  • https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-98802URI
  • https://doi.org/10.1128/aac.00251-22DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Funding agencis:United States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA R01 AI 147609  US Department of Veterans Affairs
  • The continued emergence of Neisseria gonorrhoeae isolates which are resistant to first-line antibiotics has reinvigorated interest in alternative therapies such as expanded use of gentamicin (Gen). We hypothesized that expanded use of Gen promotes emergence of gonococci with clinical resistance to this aminoglycoside. To understand how decreased susceptibility of gonococci to Gen might develop, we selected spontaneous low-level Gen-resistant (GenR) mutants (Gen MIC = 32 μg/mL) of the Gen-susceptible strain FA19. Consequently, we identified a novel missense mutation in fusA, which encodes elongation factor G (EF-G), causing an alanine (A) to valine (V) substitution at amino acid position 563 in domain IV of EF-G; the mutant allele was termed fusA2. Transformation analysis showed that fusA2 could increase the Gen MIC by 4-fold. While possession of fusA2 did not impair either in vitro gonococcal growth or protein synthesis, it did result in a fitness defect during experimental infection of the lower genital tract in female mice. Through bioinformatic analysis of whole-genome sequences of 10,634 international gonococcal clinical isolates, other fusA alleles were frequently detected, but genetic studies revealed that they could not decrease Gen susceptibility in a similar manner to fusA2. In contrast to these diverse international fusA alleles, the fusA2-encoded A563V substitution was detected in only a single gonococcal clinical isolate. We hypothesize that the rare occurrence of fusA2 in N. gonorrhoeae clinical isolates is likely due to a fitness cost during infection, but compensatory mutations which alleviate this fitness cost could emerge and promote GenR in global strains.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Dhulipala, VijayaDepartment of Microbiology and Immunology, Emory University School of Medicinegrid.471395.d, Atlanta, Georgia, USA (författare)
  • Balthazar, Jacqueline T.Department of Microbiology and Immunology, Emory University School of Medicinegrid.471395.d, Atlanta, Georgia, USA (författare)
  • Le Van, AdrianaDepartment of Microbiology and Immunology, Uniformed Services University, Bethesda, Maryland, USA (författare)
  • Begum, Afrin A.Department of Microbiology and Immunology, Uniformed Services University, Bethesda, Maryland, USA (författare)
  • Chen, Shao-ChunInstitute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, People's Republic of China; National Center for Sexually Transmitted Diseases Control, Chinese Center for Disease Control and Prevention, Nanjing, People's Republic of China (författare)
  • Read, Timothy D.Division of Infectious Diseases, Department of Medicine, Emory University School of Medicinegrid.471395.d, Atlanta, Georgia, USA; The Emory Antibiotic Resistance Center, Emory University School of Medicinegrid.471395.d, Atlanta, Georgia, USA (författare)
  • Matoga, MitchUNC Project Malawi, Lilongwe, Malawi (författare)
  • Hoffman, Irving F.Department of Medicine, University of North Carolina at Chapel Hillgrid.10698.36, Chapel Hill, North Carolina, USA (författare)
  • Golparian, Daniel,1984-Örebro universitet,Institutionen för medicinska vetenskaper,WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for STIs, Department of Laboratory Medicine, Clinical Microbiology(Swepub:oru)degn (författare)
  • Unemo, Magnus,1970-Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for STIs, Department of Laboratory Medicine, Clinical Microbiology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden; Institute for Global Health, University College London, London, United Kingdom(Swepub:oru)muo (författare)
  • Jerse, Ann E.Department of Microbiology and Immunology, Uniformed Services University, Bethesda, Maryland, USA (författare)
  • Shafer, William M.Department of Microbiology and Immunology, Emory University School of Medicinegrid.471395.d, Atlanta, Georgia, USA; The Emory Antibiotic Resistance Center, Emory University School of Medicinegrid.471395.d, Atlanta, Georgia, USA; Laboratories of Bacterial Pathogenesis, Veterans Affairs Medical Center, Decatur, Georgia, USA (författare)
  • Department of Microbiology and Immunology, Emory University School of Medicinegrid.471395.d, Atlanta, Georgia, USADepartment of Microbiology and Immunology, Uniformed Services University, Bethesda, Maryland, USA (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Antimicrobial Agents and Chemotherapy: American Society for Microbiology66:50066-48041098-6596

Internetlänk

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy