Search: L773:0927 7765 OR L773:1873 4367 >
Adsorption of MUC5B...
Adsorption of MUC5B and the role of mucins in early salivary film formation
-
- Lindh, Liselott (author)
- Malmö högskola,Odontologiska fakulteten (OD),Fakulteten för hälsa och samhälle (HS)
-
Glantz, Per-Olof (author)
-
- Carlstedt, Ingemar (author)
- Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine
-
show more...
-
- Wickström, Claes (author)
- Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine
-
- Arnebrant, Thomas (author)
- Malmö högskola,RISE,YKI – Ytkemiska institutet,Odontologiska fakulteten (OD),Fakulteten för hälsa och samhälle (HS)
-
show less...
-
(creator_code:org_t)
- Elsevier, 2002
- 2002
- English.
-
In: Colloids and Surfaces B: Biointerfaces. - : Elsevier. - 1873-4367 .- 0927-7765. ; 25:2, s. 139-146
- Related links:
-
http://dx.doi.org/10...
-
show more...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- Salivary mucins are known to play important roles in the formation of oral salivary films. The aims of the present study were to investigate the behaviour of salivary mucins at solid surfaces with different wettabilities, as well as the influence of electrolyte on the adsorption behaviour. A pure preparation of human salivary MUC5B was used together with a commercial one of bovine submaxillary mucin (BSM). Amounts adsorbed from freshly prepared solutions onto hydrophilic and hydrophobic surfaces versus time were measured in situ by ellipsometry. At low concentrations, larger amounts were adsorbed onto hydrophobic than onto hydrophilic silica indicating a higher affinity for the former surfaces. Furthermore, on hydrophilic surfaces adsorbed amounts of MUC5B and BSM show good agreement at low concentrations (< 0. 10 mg ml(-1)). However, at higher concentrations MUM adsorbed to a lower extent than BSM. At hydrophobic surfaces, isotherm shapes were similar for the two preparations, but the amounts were shifted to higher values for MUC5B. Finally, the presence of electrolyte increased adsorption and the increase was more pronounced on hydrophilic surfaces. The increased adsorption at a higher ionic strength indicates a more compact structure of the mucin due to electrostatic screening and the fact that the effect was more pronounced on the hydrophilic surfaces points to a higher relative importance of electrostatic interactions in this case. We conclude that the two mucins investigated behave in a qualitatively similar manner and show the highest affinity for hydrophobic surfaces.
Subject headings
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
Keyword
- surfaces
- salivary mucins
- ellipsometry
- adsorption
- diffusion limitation
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database