Serotonin (5-HT) and 5-HT2A receptor agonists suppress lipolysis in primary rat adipose cells

Hansson, BjörnLund University,Lunds universitet,Glukostransport och proteintrafficking,Forskargrupper vid Lunds universitet,Diabetes,Glucose Transport and Protein Trafficking,Lund University Research Groups (författare)

Serotonin (5-HT) is a biogenic monoamine that functions both as a neurotransmitter and a circulating hormone. Recently, the metabolic effects of 5-HT have gained interest and peripheral 5-HT has been proposed to influence lipid metabolism in various ways. Here, we investigated the metabolic effects of 5-HT in isolated, primary rat adipose cells. Incubation with 5-HT suppressed β-adrenergically stimulated glycerol release and decreased phosphorylation of protein kinase A (PKA)-dependent substrates, hormone sensitive lipase (Ser563) and perilipin (Ser522). The inhibitory effect of 5-HT on lipolysis enhanced the anti-lipolytic effect of insulin, but sustained in the presence of phosphodiesterase inhibitors, OPC3911 and isobuthylmethylxanthine (IBMX). The relative expression of 5-HT1A, -2B and -4 receptor class family were significantly higher in adipose tissue compared to adipose cells, whereas 5-HT1D, -2A and -7 were highly expressed in isolated adipose cells. Similar to 5-HT, 5-HT2 receptor agonists reduced lipolysis while 5-HT1 receptor agonists rather decreased non-stimulated and insulin-stimulated glucose uptake. Together, these data provide evidence of a direct effect of 5-HT on adipose cells, where 5-HT suppresses lipolysis and glucose uptake, which could contribute to altered systemic lipid- and glucose metabolism.

Benavente, Anya MedinaLund University,Lunds universitet,Diabetes - molekylär metabolism,Forskargrupper vid Lunds universitet,Diabetes - Molecular Metabolism,Lund University Research Groups(Swepub:lu)med-aym (författare)
Fryklund, Claes (författare)
Fex, MalinLund University,Lunds universitet,Diabetes - molekylär metabolism,Forskargrupper vid Lunds universitet,Diabetes - Molecular Metabolism,Lund University Research Groups(Swepub:lu)medk-mf0 (författare)
Stenkula, Karin G.Lund University,Lunds universitet,Glukostransport och proteintrafficking,Forskargrupper vid Lunds universitet,Diabetes,Glucose Transport and Protein Trafficking,Lund University Research Groups(Swepub:lu)med-ksu (författare)
Glukostransport och proteintraffickingForskargrupper vid Lunds universitet (creator_code:org_t)

Ingår i:Biochemical and Biophysical Research Communications: Elsevier BV474:2, s. 357-3630006-291X

Av författaren/redakt...: Hansson, Björn; Benavente, Anya ...; Fryklund, Claes; Fex, Malin; Stenkula, Karin ...

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinsk biotek ...; och Annan medicinsk ...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.