Antibody-based tissue profiling as a tool for clinical proteomics

• Here, we show a strategy for high-throughput antibody-based tissue profiling with the aim to create an atlas of protein expression patterns in normal human tissues and cancer tissues representing the 20 most prevalent cancer types. A set of standardized tissue microarrays (TMAs) was produced to allow for rapid screening of a multitude of different cells and tissues using immunohistochemistry. Eight TMA blocks were produced containing 48 different normal human tissues in triplicate and cancer tissue from 216 individually different tumors in duplicate. Sections from these blocks were immunohistochemically stained using five commercial and five in-house generated antibodies. Digital images for annotation of expression profiles were generated using a semiautomated approach. Five hundred seventy-six images and annotation data corresponding to a total of 30 Gbytes of data were collected for each antibody. The data presented here suggest that antibody-based profiling of protein expression in tissues can be used as a valuable tool in clinical proteomics.

Ämnesord

NATURVETENSKAP  -- Biologi -- Cellbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Cell Biology (hsv//eng)

Nyckelord

Antibody-based proteomics

Monospecific antibodies

Protein atlas

Protein epitope signature tags

Tissue microarray

CD34 antigen

cyclin A

cytokeratin 14

dyskerin

epidermal growth factor receptor 2

G protein coupled receptor

glial fibrillary acidic protein

polyadenylic acid binding protein

protein antibody

zinc finger protein

article

cancer tissue

cell screening

human

human tissue

immunohistochemistry

nucleotide sequence

priority journal

protein expression

proteomics

staining

tumor

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