WFRF:(Isaksson A)
Sökning: WFRF:(Isaksson A) > (1990-1994) > L-iduronate-rich gl...
- 1 av 6
- Föregående post
- Nästa post
- Till träfflistan
- Westergren-Thorsson, GLund University,Lunds universitet,Lungbiologi,Forskargrupper vid Lunds universitet,Lung Biology,Lund University Research Groups (författare)
L-iduronate-rich glycosaminoglycans inhibit growth of normal fibroblasts independently of serum or added growth factors
- Artikel/kapitelEngelska1993
Förlag, utgivningsår, omfång ...
- Elsevier BV,1993
Nummerbeteckningar
- LIBRIS-ID:oai:lup.lub.lu.se:92f56ce4-aacd-473f-93dd-06a521c07d5f
- https://lup.lub.lu.se/record/92f56ce4-aacd-473f-93dd-06a521c07d5fURI
- https://doi.org/10.1006/excr.1993.1124DOI
Kompletterande språkuppgifter
- Språk:engelska
- Sammanfattning på:engelska
Ingår i deldatabas
- SwePubSwePub
Klassifikation
Anmärkningar
- The effects of various glycosaminoglycans (GAGs) on the growth rate of normal fibroblasts and a fibrosarcoma cell line (HT 1080) were examined. Cells were grown in 96-well microplates in the absence or presence of serum mitogens, epidermal (EGF), platelet-derived (PDGF), acidic fibroblast (aFGF), or basic fibroblast growth factor (bFGF). Cell number was measured by using crystal violet to stain cell nuclei (Westergren-Thorsson, G., Onnervik, P.-O., Fransson, L.-A., and Malmström, A. J. Cell. Phys. 147, 523-530, 1991) and also by using a Coulter counter. In the presence of serum mitogens, L-iduronate (IdoA)-rich GAGs, such as dermatan sulfate, heparin, and highly sulfated heparan sulfate, inhibited proliferation of normal cells (25-35%), whereas HT 1080 cells were unaffected or slightly stimulated. Ham's F-12 supplemented with insulin and transferrin but without growth factors was able to support growth of both cell types. Under these conditions, the IdoA-rich GAGs still suppressed growth of normal cells (40-55%), whereas HT 1080 cells again responded poorly. When growth factors were added proliferation of normal fibroblasts was further stimulated, EGF being the most effective. In the presence of either EGF, PDGF, or bFGF, IdoA-rich GAGs had a sustained inhibitory effect on normal fibroblasts (30-50% at concentrations at or above 10 micrograms/ml). However, in the presence of aFGF, both IdoA-rich and IdoA-poor heparan sulfates enhanced growth (nearly twofold after prolonged exposure) suggesting a stabilization of this growth factor. In general, IdoA-rich GAGs appear to inhibit proliferation of normal cells irrespective of the type of growth factor used. Therefore, GAGs are likely to act directly on cell-derived regulatory components, either before or after internalization. As fibrosarcoma cells were much less sensitive to growth inhibition, they may contain altered receptors for GAGs.
Ämnesord och genrebeteckningar
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
- Blood Proteins
- Cell Division
- Cells, Cultured
- Dermatan Sulfate
- Epidermal Growth Factor
- Fibroblast Growth Factor 1
- Fibroblast Growth Factor 2
- Fibroblasts
- Fibrosarcoma
- Glycosaminoglycans
- Growth Inhibitors
- Growth Substances
- Heparitin Sulfate
- Humans
- Iduronic Acid
- Lung
- Mitogens
- Platelet-Derived Growth Factor
- Tumor Cells, Cultured
- Journal Article
- Research Support, Non-U.S. Gov't
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Persson, SLund University (författare)
- Isaksson, ALund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)kkem-ais (författare)
- Önnervik, Per-OlaLund University (författare)
- Malmström, ALund University,Lunds universitet,Matrixbiologi,Forskargrupper vid Lunds universitet,Matrix Biology,Lund University Research Groups(Swepub:lu)medk-ama (författare)
- Fransson, L ALund University,Lunds universitet,Glykobiologigruppen,Forskargrupper vid Lunds universitet,Glycobiology,Lund University Research Groups(Swepub:lu)medk-laf (författare)
- LungbiologiForskargrupper vid Lunds universitet (creator_code:org_t)
Sammanhörande titlar
- Ingår i:Experimental Cell Research: Elsevier BV206:1, s. 9-930014-4827
Internetlänk
Hitta via bibliotek
- Experimental Cell Research (Sök värdpublikationen i LIBRIS)
Till lärosätets databas
- 1 av 6
- Föregående post
- Nästa post
- Till träfflistan
Hitta mer i SwePub
- Av författaren/redakt...
- Westergren-Thors ...
- Persson, S
- Isaksson, A
- Önnervik, Per-Ol ...
- Malmström, A
- Fransson, L A
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
- MEDICIN OCH HÄLS ...
- och Medicinska och f ...
- och Cell och molekyl ...
- Artiklar i publikationen
- Experimental Cel ...
- Av lärosätet
- Lunds universitet
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
