The transcriptome of the avian malaria parasite Plasmodium ashfordi displays host-specific gene expression

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Videvall, ElinLund University,Lunds universitet,MEMEG,Biologiska institutionen,Naturvetenskapliga fakulteten,Molekylär ekologi och evolution,Forskargrupper vid Lunds universitet,Department of Biology,Faculty of Science,Molecular Ecology and Evolution Lab,Lund University Research Groups,Department of Biology Lund University Sölvegatan 37 SE‐22362 Lund Sweden (författare)

The transcriptome of the avian malaria parasite Plasmodium ashfordi displays host-specific gene expression

Artikel/kapitelEngelska2017

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2017-04-08
Wiley,2017

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LIBRIS-ID:oai:lup.lub.lu.se:980dab26-f1cd-4ebb-9647-56bc23b52e87
https://lup.lub.lu.se/record/980dab26-f1cd-4ebb-9647-56bc23b52e87URI
https://doi.org/10.1111/mec.14085DOI
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-517155URI

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Språk:engelska
Sammanfattning på:engelska &svenska

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Ämneskategori:ref swepub-contenttype

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Malaria parasites (Plasmodium spp.) include some of the world's most widespread and virulent pathogens. Our knowledge of the molecular mechanisms these parasites use to invade and exploit hosts other than mice and primates is, however, extremely limited. It is therefore imperative to characterize transcriptome-wide gene expression from non-model malaria parasites and how this varies across host individuals. Here, we used high-throughput Illumina RNA-sequencing on blood from wild-caught Eurasian siskins experimentally infected with a clonal strain of the avian malaria parasite Plasmodium ashfordi (lineage GRW2). By using a multi-step approach to filter out host transcripts, we successfully assembled the blood-stage transcriptome of P. ashfordi. A total of 11 954 expressed transcripts were identified, and 7 860 were annotated with protein information. We quantified gene expression levels of all parasite transcripts across three hosts during two infection stages – peak and decreasing parasitemia. Interestingly, parasites from the same host displayed remarkably similar expression profiles during different infection stages, but showed large differences across hosts, indicating that P. ashfordi may adjust its gene expression to specific host individuals. We further show that the majority of transcripts are most similar to the human parasite Plasmodium falciparum, and a large number of red blood cell invasion genes were discovered, suggesting evolutionary conserved invasion strategies between mammalian and avian Plasmodium. The transcriptome of P. ashfordi and its host-specific gene expression advances our understanding of Plasmodium plasticity and is a valuable resource as it allows for further studies analysing gene evolution and comparisons of parasite gene expression.
Malaria parasites (Plasmodium spp.) include some of the world's most widespread and virulent pathogens. Our knowledge of the molecular mechanisms these parasites use to invade and exploit hosts other than mice and primates is, however, extremely limited. It is therefore imperative to characterize transcriptome-wide gene expression from non-model malaria parasites and how this varies across host individuals. Here, we used high-throughput Illumina RNA-sequencing on blood from wild-caught Eurasian siskins experimentally infected with a clonal strain of the avian malaria parasite Plasmodium ashfordi (lineage GRW2). By using a multi-step approach to filter out host transcripts, we successfully assembled the blood-stage transcriptome of P. ashfordi. A total of 11 954 expressed transcripts were identified, and 7 860 were annotated with protein information. We quantified gene expression levels of all parasite transcripts across three hosts during two infection stages – peak and decreasing parasitemia. Interestingly, parasites from the same host displayed remarkably similar expression profiles during different infection stages, but showed large differences across hosts, indicating that P. ashfordi may adjust its gene expression to specific host individuals. We further show that the majority of transcripts are most similar to the human parasite Plasmodium falciparum, and a large number of red blood cell invasion genes were discovered, suggesting evolutionary conserved invasion strategies between mammalian and avian Plasmodium. The transcriptome of P. ashfordi and its host-specific gene expression advances our understanding of Plasmodium plasticity and is a valuable resource as it allows for further studies analysing gene evolution and comparisons of parasite gene expression.

Ämnesord och genrebeteckningar

NATURVETENSKAP Biologi Zoologi hsv//swe
NATURAL SCIENCES Biological Sciences Zoology hsv//eng
NATURVETENSKAP Biologi Genetik hsv//swe
NATURAL SCIENCES Biological Sciences Genetics hsv//eng
RNA-seq
host-parasite interaction

Biuppslag (personer, institutioner, konferenser, titlar ...)

Cornwallis, CharlieLund University,Lunds universitet,MEMEG,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science,Department of Biology Lund University Sölvegatan 37 SE‐22362 Lund Sweden(Swepub:lu)biol-ccn (författare)
Ahrén, DagLund University,Lunds universitet,MEMEG,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science,Department of Biology Lund University Sölvegatan 37 SE‐22362 Lund Sweden;National Bioinformatics Infrastructure Sweden (NBIS) Lund University Sölvegatan 37 SE‐22362 Lund Sweden(Swepub:lu)mbek-dah (författare)
Palinauskas, VaidasState Scientific Institute Nature Research Centre,Institute of Ecology Nature Research Centre Akademijos 2 LT‐08412 Vilnius Lithuania (författare)
Valkiunas, GediminasState Scientific Institute Nature Research Centre,Institute of Ecology Nature Research Centre Akademijos 2 LT‐08412 Vilnius Lithuania (författare)
Hellgren, OlofLund University,Lunds universitet,MEMEG,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science,Department of Biology Lund University Sölvegatan 37 SE‐22362 Lund Sweden(Swepub:lu)zooe-ohe (författare)
MEMEGBiologiska institutionen (creator_code:org_t)

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Ingår i:Molecular Ecology: Wiley0962-10831365-294X

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Av författaren/redakt...: Videvall, Elin; Cornwallis, Char ...; Ahrén, Dag; Palinauskas, Vai ...; Valkiunas, Gedim ...; Hellgren, Olof

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NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Zoologi

NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Genetik

Artiklar i publikationen: Molecular Ecolog ...

Av lärosätet: Lunds universitet; Uppsala universitet

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