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Workshop report on ...
Workshop report on the extraction of foetal DNA from maternal plasma
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Legler, Tobias J. (author)
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Liu, Zhong (author)
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Mavrou, Ariadni (author)
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Finning, Kirstin (author)
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Hromadnikova, Ilona (author)
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Galbiati, Silvia (author)
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Meaney, Cathy (author)
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Hulten, Maj A. (author)
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Crea, Francesco (author)
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- Olsson, Martin L (author)
- Lund University,Lunds universitet,Avdelningen för hematologi och transfusionsmedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Transfusionsmedicin,Forskargrupper vid Lunds universitet,Division of Hematology and Transfusion Medicine,Department of Laboratory Medicine,Faculty of Medicine,Transfusion Medicine,Lund University Research Groups
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Maddocks, Deborah G. (author)
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Huang, Dorothy (author)
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Fisher, Sylvia Armstrong (author)
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Sprenger-Haussels, Markus (author)
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Soussan, Aicha Ait (author)
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van der Schoot, C. Ellen (author)
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(creator_code:org_t)
- 2007
- 2007
- English.
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In: Prenatal Diagnosis. - : Wiley. - 1097-0223 .- 0197-3851. ; 27:9, s. 824-829
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
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- Objective Cell free foetal DNA (cff DNA) extracted from maternal plasma is now recognized as a potential source for prenatal diagnosis but the methodology is currently not well standardized. To evaluate different manual and automated DNA extraction methods with a view to developing standards, an International Workshop was performed. Methods Three plasma pools from RhD-negative pregnant women, a DNA standard, real-time-PCR protocol, primers and probes for RHD were sent to 12 laboratories and also to one company (Qiagen, Hilden, Germany). In pre-tests, pool 3 showed a low cff DNA concentration, pool I showed a higher concentration and pool 2 an intermediate concentration. Results The QIAamp DSP Virus Kit, the High Pure PCR Template Preparation Kit, an in-house protocol using the QIAamp DNA Blood Mini Kit, the CST genomic DNA purification kit, the Magna Pure LC, the MDx, the M48, the EZl and an in-house protocol using magnetic beads for manual and automated extraction were the methods that were able to reliably detect foetal RHD. The best results were obtained with the QIAamp DSP Virus Kit. The QIAamp DNA Blood Mini Kit showed very comparable results in laboratories that followed the manufacturer's protocol and started with >= 500 mu L plasma. One participant using the QIAamp DNA Blood Midi Kit failed to detect reliably RHD in pool 3. Conclusions This workshop initiated a standardization process for extraction of cff DNA in maternal plasma. The highest yield was obtained by the QIAamp DSP Virus Kit, a result that will be evaluated in more detail in future studies. Copyright (c) 2007 John Wiley & Sons, Ltd.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reproduktionsmedicin och gynekologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Obstetrics, Gynaecology and Reproductive Medicine (hsv//eng)
Keyword
- cell-free foetal DNA
- non-invasive prenatal diagnosis
- maternal plasma
- genotyping
- RhD
- sensitivity
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Legler, Tobias J ...
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Liu, Zhong
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Mavrou, Ariadni
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Finning, Kirstin
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Hromadnikova, Il ...
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Galbiati, Silvia
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show more...
-
Meaney, Cathy
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Hulten, Maj A.
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Crea, Francesco
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Olsson, Martin L
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Maddocks, Debora ...
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Huang, Dorothy
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Fisher, Sylvia A ...
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Sprenger-Haussel ...
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Soussan, Aicha A ...
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van der Schoot, ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Obstetrics Gynae ...
- Articles in the publication
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Prenatal Diagnos ...
- By the university
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Lund University