Search: WFRF:(Monteiro Patrícia)
> (2010-2014) >
Nitric Oxide Stimul...
Nitric Oxide Stimulates the Proliferation of Neural Stem Cells Bypassing the Epidermal Growth Factor Receptor
-
Carreira, Bruno Pereira (author)
-
Morte, Maria Ines (author)
-
Inacio, Angela (author)
-
show more...
-
Costa, Gabriel (author)
-
Rosmaninho-Salgado, Joana (author)
-
Agasse, Fabienne (author)
-
Carmo, Analia (author)
-
Couceiro, Patricia (author)
-
- Brundin, Patrik (author)
- Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine
-
Ambrosio, Antonio Francisco (author)
-
Carvalho, Caetana Monteiro (author)
-
Araujo, Ines Maria (author)
-
show less...
-
(creator_code:org_t)
- 2010-05-18
- 2010
- English.
-
In: Stem Cells. - : Oxford University Press (OUP). - 1549-4918 .- 1066-5099. ; 28:7, s. 1219-1230
- Related links:
-
http://dx.doi.org/10...
-
show more...
-
https://stemcellsjou...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Nitric oxide (NO) was described to inhibit the proliferation of neural stem cells. Some evidence suggests that NO, under certain conditions, can also promote cell proliferation, although the mechanisms responsible for a potential proliferative effect of NO in neural stem cells have remained unaddressed. In this work, we investigated and characterized the proliferative effect of NO in cell cultures obtained from the mouse subventricular zone. We found that the NO donor NOC-18 (10 mu M) increased cell proliferation, whereas higher concentrations (100 mu M) inhibited cell proliferation. Increased cell proliferation was detected rapidly following exposure to NO and was prevented by blocking the mitogen-activated kinase (MAPK) pathway, independently of the epidermal growth factor (EGF) receptor. Downstream of the EGF receptor, NO activated p21Ras and the MAPK pathway, resulting in a decrease in the nuclear presence of the cyclin-dependent kinase inhibitor I, p27(KIP1), allowing for cell cycle progression. Furthermore, in a mouse model that shows increased proliferation of neural stem cells in the hippocampus following seizure injury, we observed that the absence of inducible nitric oxide synthase (iNOS(-/-) mice) prevented the increase in cell proliferation observed following seizures in wild-type mice, showing that NO from iNOS origin is important for increased cell proliferation following a brain insult. Overall, we show that NO is able to stimulate the proliferation of neural stem cells bypassing the EGF receptor and promoting cell division. Moreover, under pathophysiological conditions in vivo, NO from iNOS origin also promotes proliferation in the hippocampus. STEM CELLS 2010:28:1219-1230
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- factor receptor
- Epidermal growth
- Cell proliferation
- Nitric oxide
- Neural stem cells
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
-
Stem Cells
(Search for host publication in LIBRIS)
To the university's database
- By the author/editor
-
Carreira, Bruno ...
-
Morte, Maria Ine ...
-
Inacio, Angela
-
Costa, Gabriel
-
Rosmaninho-Salga ...
-
Agasse, Fabienne
-
show more...
-
Carmo, Analia
-
Couceiro, Patric ...
-
Brundin, Patrik
-
Ambrosio, Antoni ...
-
Carvalho, Caetan ...
-
Araujo, Ines Mar ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
- Articles in the publication
-
Stem Cells
- By the university
-
Lund University