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- Kasetty, GopinathLund University,Lunds universitet,Lungmedicin, allergologi och palliativ medicin,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Respiratory Medicine, Allergology, and Palliative Medicine,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund (author)
Anti-endotoxic and antibacterial effects of a dermal substitute coated with host defense peptides.
- Article/chapterEnglish2015
Publisher, publication year, extent ...
- Elsevier BV,2015
- electronicrdacarrier
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- LIBRIS-ID:oai:lup.lub.lu.se:9cc1215b-5d8c-40dc-8919-025bc4ce0aff
- https://lup.lub.lu.se/record/5341307URI
- https://doi.org/10.1016/j.biomaterials.2015.02.111DOI
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- Language:English
- Summary in:English
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- Biomaterials used during surgery and wound treatment are of increasing importance in modern medical care. In the present study we set out to evaluate the addition of thrombin-derived host defense peptides to human acellular dermis (hAD, i.e. epiflex(®)). Antimicrobial activity of the functionalized hAD was demonstrated using radial diffusion and viable count assays against Gram-negative Escherichia coli, Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus bacteria. Electron microscopy analyses showed that peptide-mediated bacterial killing led to reduced hAD degradation. Furthermore, peptide-functionalized hAD displayed endotoxin-binding activity in vitro, as evidenced by inhibition of NF-κB activation in human monocytic cells (THP-1 cells) and a reduction of pro-inflammatory cytokine production in whole blood in response to lipopolysaccharide stimulation. The dermal substitute retained its anti-endotoxic activity after washing, compatible with results showing that the hAD bound a significant amount of peptide. Furthermore, bacteria-induced contact activation was inhibited by peptide addition to the hAD. E. coli infected hAD, alone, or after treatment with the antiseptic substance polyhexamethylenebiguanide (PHMB), yielded NF-κB activation in THP-1 cells. The activation was abrogated by peptide addition. Thus, thrombin-derived HDPs should be of interest in the further development of new biomaterials with combined antimicrobial and anti-endotoxic functions for use in surgery and wound treatment.
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- Kalle, MartinaLund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine(Swepub:lu)med-mtk (author)
- Mörgelin, MatthiasLund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)medk-mmn (author)
- Brune, Jan ClaasLund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)stem-jbn (author)
- Schmidtchen, ArturLund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)derm-asc (author)
- Lungmedicin, allergologi och palliativ medicinSektion II (creator_code:org_t)
Related titles
- In:Biomaterials: Elsevier BV53, s. 415-4251878-59050142-9612
Internet link
- https://portal.research.lu.se/files/1925841/8227743FULLTEXT
- http://www.ncbi.nlm.nih.gov/pubmed/25890739?dopt=AbstractFULLTEXT
- http://dx.doi.org/10.1016/j.biomaterials.2015.02.111FULLTEXT
- https://doi.org/10.1016/j.biomaterials.2015.02.111
- https://lup.lub.lu.se/record/5341307
- https://doi.org/10.1016/j.biomaterials.2015.02.111
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