WFRF:(Zhang H.)
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- Leinonen, J.Helsinki University Central Hospital (author)
Reactivity of anti-PSA monoclonal antibodies with recombinant human kallikrein-2
- Article/chapterEnglish1999
Publisher, publication year, extent ...
- Springer Science and Business Media LLC,1999
Numbers
- LIBRIS-ID:oai:lup.lub.lu.se:9e225900-e09f-4451-b9ea-694c73b69afb
- https://lup.lub.lu.se/record/9e225900-e09f-4451-b9ea-694c73b69afbURI
- https://doi.org/10.1159/000056528DOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- Seventy-nine monoclonal antibodies submitted to the ISOBM TD-3 PSA Workshop were tested for their reactivity with recombinant human kallikrein-2 (rhK2). A sandwich immunofluorometric assay using polyclonal antiprostate- specific antigen (PSA) antiserum-coated plates was used to capture rhK2 and subsequently the test antibody. The response of each test antibody was compared with 3 reference antibodies (H50, H117 and 5E4) known to react with hK2. Nine antibodies from the workshop panel failed to react with purified PSA and rhK2 in this assay and were subsequently excluded. From the remaining panel of antibodies, 11/70 showed strong reactivity with rhK2, 9/70 showed weak reactivity with rhK2, while 50/70 antibodies did not react with rhK2 in this assay format. All antibodies binding to rhK2 recognized both free and complexed PSA.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Läkemedelskemi hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Medicinal Chemistry hsv//eng
- Human kallikrein-2
- ISOBM
- Monoclonal antibodies
- Prostate-specific antigen
- Recombinant kallikrein
- TD-3
Added entries (persons, corporate bodies, meetings, titles ...)
- Leinimaa, M.Helsinki University Central Hospital (author)
- Zhang, W. M.Helsinki University Central Hospital (author)
- Piironen, T.University of Turku (author)
- Pettersson, K.University of Turku (author)
- Lilja, H.Lund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups,Skåne University Hospital(Swepub:lu)klke-hli (author)
- Dowell, B.Abbott Laboratories, US (author)
- Stenman, U. H.Helsinki University Central Hospital (author)
- Helsinki University Central HospitalUniversity of Turku (creator_code:org_t)
Related titles
- In:Tumor Biology: Springer Science and Business Media LLC20:SUPPL. 1, s. 35-371010-42831423-0380
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