Search: WFRF:(Alsved Malin) >
Effect of Aerosoliz...
Effect of Aerosolization and Drying on the Viability of Pseudomonas syringae Cells
-
- Alsved, Malin (author)
- Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Ergonomi och aerosolteknologi,Institutionen för designvetenskaper,Institutioner vid LTH,Other operations, LTH,Faculty of Engineering, LTH,Ergonomics and Aerosol Technology,Department of Design Sciences,Departments at LTH,Faculty of Engineering, LTH
-
- Holm, Stine (author)
- Aarhus University
-
- Christiansen, Sigurd (author)
- Aarhus University
-
show more...
-
- Smidt, Mads (author)
- Aarhus University
-
- Ling, Meilee (author)
- Aarhus University
-
- Boesen, Thomas (author)
- Aarhus University
-
- Finster, Kai (author)
- Aarhus University
-
- Bilde, Merete (author)
- Aarhus University
-
- Löndahl, Jakob (author)
- Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Ergonomi och aerosolteknologi,Institutionen för designvetenskaper,Institutioner vid LTH,Other operations, LTH,Faculty of Engineering, LTH,Ergonomics and Aerosol Technology,Department of Design Sciences,Departments at LTH,Faculty of Engineering, LTH
-
- Šantl-Temkiv, Tina (author)
- Aarhus University
-
show less...
-
(creator_code:org_t)
- 2018-12-18
- 2018
- English.
-
In: Frontiers in Microbiology. - : Frontiers Media SA. - 1664-302X. ; 9
- Related links:
-
http://dx.doi.org/10... (free)
-
show more...
-
https://www.frontier...
-
https://lup.lub.lu.s...
-
https://doi.org/10.3...
-
show less...
Abstract
Subject headings
Close
- Airborne dispersal of microorganisms influences their biogeography, gene flow, atmospheric processes, human health and transmission of pathogens that affect humans, plants and animals. The extent of their impact depends essentially on cell-survival rates during the process of aerosolization. A central factor for cell-survival is water availability prior to and upon aerosolization. Also, the ability of cells to successfully cope with stress induced by drying determines their chances of survival. In this study, we used the ice-nucleation active, plant pathogenic Pseudomonas syringae strain R10.79 as a model organism to investigate the effect of drying on cell survival. Two forms of drying were simulated: drying of cells in small droplets aerosolized from a wet environment by bubble bursting and drying of cells in large droplets deposited on a surface. For drying of cells both in aerosol and surface droplets, the relative humidity (RH) was varied in the range between 10 and 90%. The fraction of surviving cells was determined by live/dead staining followed by flow cytometry. We also evaluated the effect of salt concentration in the water droplets on the survival of drying cells by varying the ionic strength between 0 and 700 mM using NaCl and sea salt. For both aerosol and surface drying, cell survival increased with decreasing RH (p < 0.01), and for surface drying, survival was correlated with increasing salt concentration (p < 0.001). Imaging cells with TEM showed shrunk cytoplasm and cell wall damage for a large fraction of aerosolized cells. Ultimately, we observed a 10-fold higher fraction of surviving cells when dried as aerosol compared to when dried on a surface. We conclude that the conditions, under which cells dry, significantly affect their survival and thus their success to spread through the atmosphere and colonize new environments as well as their ability to affect atmospheric processes.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Annan teknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Other Engineering and Technologies (hsv//eng)
Keyword
- Bioaerosols
- Aerosolization
- Pseudomonas syringae
- Bubble bursting
- Drying
- Ice nucleation activity
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database
- By the author/editor
-
Alsved, Malin
-
Holm, Stine
-
Christiansen, Si ...
-
Smidt, Mads
-
Ling, Meilee
-
Boesen, Thomas
-
show more...
-
Finster, Kai
-
Bilde, Merete
-
Löndahl, Jakob
-
Šantl-Temkiv, Ti ...
-
show less...
- About the subject
-
- ENGINEERING AND TECHNOLOGY
-
ENGINEERING AND ...
-
and Other Engineerin ...
- Articles in the publication
-
Frontiers in Mic ...
- By the university
-
Lund University