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Production of an am...
Production of an amino acid sequence-specific antiserum against human amyloid A (AA) and serum amyloid A (SAA) protein
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- Grubb, Anders (author)
- Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Cystatin C, njursjukdom, amyloidos och antibiotika,Forskargrupper vid Lunds universitet,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine,Cystatin C, renal disease, amyloidosis and antibiotics,Lund University Research Groups,Skåne University Hospital
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- Löfberg, Helge (author)
- Lund University,Lunds universitet,Patologi, Malmö,Forskargrupper vid Lunds universitet,Pathology, Malmö,Lund University Research Groups
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- Thysell, Hans (author)
- Lund University,Lunds universitet,Njurmedicin,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Nephrology,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Ljunggren, Lennart (author)
- Lund University
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- Olsson, Thomas (author)
- Gambro AB
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- Skinner, Martha (author)
- Boston University
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- Shirahama, Tsuranobu (author)
- Boston University
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- Cohen, Alan S (author)
- Boston University
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(creator_code:org_t)
- 1987
- 1987
- English 8 s.
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In: Scandinavian Journal of Clinical and Laboratory Investigation. - 0036-5513. ; 47:6, s. 619-626
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Abstract
Subject headings
Close
- The hydrophilic nonapeptide Ser-Asp-Ala-Arg-Glu-Asn-Ile-Gln-Arg, identical with residues 59-67 of human amyloid protein A (AA) and serum amyloid protein A (SAA), was covalently bound via its carboxyl-terminal end to the carrier-protein keyhole limpet haemocyanin. The complex was injected subcutaneously into ten rabbits. All rabbits produced antisera which, unabsorbed, were specific for AA and SAA. The antisera and their isolated peptide specific antibodies were performance-tested and found to be excellent for demonstration of AA and SAA in immunoblotting and immunohistochemical techniques but unsuitable for immunoprecipitation. Since it is difficult to produce AA- and SAA-specific antisera by procedures earlier described and commercial supplies of good such reagents are unavailable, the easy production of sequence-specific such antisera will facilitate more extended studies of the corresponding antigens for diagnostic and scientific purposes.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Amino Acid Sequence
- Animals
- Electrophoresis, Polyacrylamide Gel
- Immune Sera/analysis
- Immunization
- Immunohistochemistry
- Molecular Sequence Data
- Rabbits
- Serum Amyloid A Protein/administration & dosage
Publication and Content Type
- art (subject category)
- ref (subject category)
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