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The inhibition of a...
The inhibition of ammonium uptake in excised birch (Betula pendula) roots by batatasin-III.
- Article/chapterEnglish2001
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Numbers
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LIBRIS-ID:oai:lup.lub.lu.se:acfee8eb-b834-4581-a2e8-775011bc6e8a
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https://lup.lub.lu.se/record/108730URI
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https://doi.org/10.1034/j.1399-3054.2001.1130310.xDOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:art swepub-publicationtype
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Subject category:ref swepub-contenttype
Notes
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In northern Sweden, plants growing in association with the clonal dwarf shrub Empetrum hermaphroditum usually exhibit limited growth and are N-depleted. Previous studies suggest that this negative effect by E. hermaphroditum may be explained, at least in part, by the release of phenolic compounds, particularly the dihydrostilbene, batatasin-III from foliage to soil. In the present work, we investigated whether batatasin-III has the potential to interfere with NH4+ uptake in birch (Betula pendula) roots. Excised birch roots were exposed to batatasin-III during brief periods in 15NH4+ solutions, and then analyzed for labeled N. Batatasin-III inhibited N-NH4+ uptake by 28, 89 and 95% compared with the control, when roots were treated with 0.1, 1.0 and 2.8 mM of batatasin-III, respectively. The effect of 1.0-mM batatasin-III was greater at pH 4.2 than at pH 6.8. In addition, the inhibition of N-NH4+ uptake by batatasin-III was not reversed after rinsing the roots in water and transferring them to a batatasin-III free solution. Furthermore, birch seedlings immersed in a 1.0-mM batatasin-III solution for 2 h, and then replanted in pots with soil, had decreased growth, such that 10 weeks after treatment, the dry mass of both shoots and roots was reduced by 74 and 73%, respectively, compared with control seedlings. This suggests that a brief exposure to batatasin-III may have a long-term inhibitory effect on whole plant growth. Using plasma membrane vesicles isolated from easily extractable spinach (Spinacia oleracea) leaves, it was found that batatasin-III strongly inhibited proton pumping in isolated plasma membrane vesicles, while it only slightly inhibited ATP hydrolytic activity. The uncoupling of proton pumping from ATP hydrolytic activity suggests that batatasin-III disturbs membrane integrity. This hypothesis was further supported by a greater efflux of ions from birch roots immersed in a batatasin-III solution than from roots in a control solution.
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Sommarin, MarianneLund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)pbio-mso
(author)
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Nilsson, Marie-Charlotte
(author)
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Munson, Alison D.
(author)
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Margolis, Hank A.
(author)
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Biokemi och StrukturbiologiCentrum för Molekylär Proteinvetenskap
(creator_code:org_t)
Related titles
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In:Physiologia Plantarum: Wiley113:3, s. 368-3760031-9317
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