id:"swepub:oai:lup.lub.lu.se:b22f0256-499d-45e8-a5ff-18dfc0cb5890"
Sökning: id:"swepub:oai:lup.lub.lu.se:b22f0256-499d-45e8-a5ff-18dfc0cb5890" > Stopped-flow Light ...
- 1 av 1
- Föregående post
- Nästa post
- Till träfflistan
- Gena, PatriziaUniversity of Bari Aldo Moro (författare)
Stopped-flow Light Scattering Analysis of Red Blood Cell Glycerol Permeability
- Artikel/kapitelEngelska2020
Förlag, utgivningsår, omfång ...
- 2020
Nummerbeteckningar
- LIBRIS-ID:oai:lup.lub.lu.se:b22f0256-499d-45e8-a5ff-18dfc0cb5890
- https://lup.lub.lu.se/record/b22f0256-499d-45e8-a5ff-18dfc0cb5890URI
- https://doi.org/10.21769/BioProtoc.3723DOI
Kompletterande språkuppgifter
- Språk:engelska
- Sammanfattning på:engelska
Ingår i deldatabas
- SwePubSwePub
Klassifikation
Anmärkningar
- Stopped-Flow Light Scattering (SFLS) is a method devised to analyze the kinetics of fast chemical reactions that result in a significant change of the average molecular weight and/or in the shape of the reaction substrates. Several modifications of the original stopped-flow system have been made leading to a significant extension of its technical applications. One of these modifications allows the biophysical characterization of the water and solute permeability of biological and artificial membranes. Here, we describe a protocol of SFLS to measure the glycerol permeability of isolated human red blood cells (RBCs) and evaluate the pharmacokinetics properties (selectivity and potency) of isoform-specific inhibitors of AQP3, AQP7 and AQP9, three mammalian aquaglyceroporins allowing transport of glycerol across membranes. Suspensions of RBCs (1% hematocrit) are exposed to an inwardly directed gradient of 100 mM glycerol in a SFLS apparatus at 20 °C and the resulting changes in scattered light intensity are recorded at a monochromatic wavelength of 530 nm for 120 s. The SFLS apparatus is set up to have a dead time of 1.6-ms and 99% mixing efficiency in less than 1 ms. Data are fitted to a single exponential function and the related time constant (τ, seconds) of the cell-swelling phase of light scattering corresponding to the osmotic movement of water that accompanies the entry of glycerol into erythrocytes is measured. The coefficient of glycerol permeability (Pgly, cm/s) of RBCs is calculated with the following equation: Pgly = 1/[(S/V)τ] where τ (s) is the fitted exponential time constant and S/V is the surface-to-volume ratio (cm-1) of the analyzed RBC specimen. Pharmacokinetics of the isoform-specific inhibitors of AQP3, AQP7 and AQP9 are assessed by evaluating the extent of RBC Pgly values resulting after the exposure to serial concentrations of the blockers.
Ämnesord och genrebeteckningar
- NATURVETENSKAP Kemi Fysikalisk kemi hsv//swe
- NATURAL SCIENCES Chemical Sciences Physical Chemistry hsv//eng
- Aquaglyceroporins
- Aquaporin inhibitors
- Erythrocytes
- Glycerol membrane permeability
- Stopped-flow light scattering
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Portincasa, PieroBari University Hospital (författare)
- Matera, SabinoUniversity of Bari Aldo Moro(Swepub:lu)sa7601ma (författare)
- Sonntag, YonathanLund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)bioc-yas (författare)
- Rützler, MichaelLund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Apoglyx AB(Swepub:lu)bioc-mrr (författare)
- Calamita, GiuseppeUniversity of Bari Aldo Moro (författare)
- University of Bari Aldo MoroBari University Hospital (creator_code:org_t)
Sammanhörande titlar
- Ingår i:Bio-protocol10:162331-8325
Internetlänk
- 1 av 1
- Föregående post
- Nästa post
- Till träfflistan
Hitta mer i SwePub
- Av författaren/redakt...
- Gena, Patrizia
- Portincasa, Pier ...
- Matera, Sabino
- Sonntag, Yonatha ...
- Rützler, Michael
- Calamita, Giusep ...
- Om ämnet
-
- NATURVETENSKAP
- NATURVETENSKAP
- och Kemi
- och Fysikalisk kemi
- Artiklar i publikationen
- Bio-protocol
- Av lärosätet
- Lunds universitet
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
