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Long-term calcium i...
Long-term calcium imaging reveals functional development in hiPSC-derived cultures comparable to human but not rat primary cultures
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- Estévez-Priego, Estefanía (author)
- University of Barcelona
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- Moreno-Fina, Martina (author)
- University of Barcelona
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- Monni, Emanuela (author)
- Lund University,Lunds universitet,Neural stamcellsbiologi och terapi,Forskargrupper vid Lunds universitet,Stem Cells & Restorative Neurology,Neural stem cell biology and therapy,Lund University Research Groups
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- Kokaia, Zaal (author)
- Lund University,Lunds universitet,Neural stamcellsbiologi och terapi,Forskargrupper vid Lunds universitet,Stem Cells & Restorative Neurology,Neural stem cell biology and therapy,Lund University Research Groups
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- Soriano, Jordi (author)
- University of Barcelona
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- Tornero, Daniel (author)
- Institutd' Investigacions Biomèdiques August Pi iSunyer (IDIBAPS),University of Barcelona
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(creator_code:org_t)
- Elsevier BV, 2023
- 2023
- English 15 s.
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In: Stem Cell Reports. - : Elsevier BV. - 2213-6711. ; 18:1, s. 205-219
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http://dx.doi.org/10... (free)
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Abstract
Subject headings
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- Models for human brain-oriented research are often established on primary cultures from rodents, which fails to recapitulate cellular specificity and molecular cues of the human brain. Here we investigated whether neuronal cultures derived from human induced pluripotent stem cells (hiPSCs) feature key advantages compared with rodent primary cultures. Using calcium fluorescence imaging, we tracked spontaneous neuronal activity in hiPSC-derived, human, and rat primary cultures and compared their dynamic and functional behavior as they matured. We observed that hiPSC-derived cultures progressively changed upon development, exhibiting gradually richer activity patterns and functional traits. By contrast, rat primary cultures were locked in the same dynamic state since activity onset. Human primary cultures exhibited features in between hiPSC-derived and rat primary cultures, although traits from the former predominated. Our study demonstrates that hiPSC-derived cultures are excellent models to investigate development in neuronal assemblies, a hallmark for applications that monitor alterations caused by damage or neurodegeneration.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- calcium imaging
- functional connectivity
- human cellular models
- human iPSCs
- neural stem cells
- neuronal networks
Publication and Content Type
- art (subject category)
- ref (subject category)
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