Sökning: WFRF:(Pfefferle Aline)
> (2014) >
Human anaplastic th...
-
Wennerberg, ErikKarolinska Institute
(författare)
Human anaplastic thyroid carcinoma cells are sensitive to NK cell-mediated lysis via ULBP2/5/6 and chemoattract NK cells
- Artikel/kapitelEngelska2014
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
-
LIBRIS-ID:oai:lup.lub.lu.se:b4cd32c7-d4ce-4adb-aed1-6cf45caaa3d4
-
https://lup.lub.lu.se/record/b4cd32c7-d4ce-4adb-aed1-6cf45caaa3d4URI
-
https://doi.org/10.1158/1078-0432.CCR-14-0291DOI
-
http://kipublications.ki.se/Default.aspx?queryparsed=id:130137501URI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:art swepub-publicationtype
-
Ämneskategori:ref swepub-contenttype
Anmärkningar
-
PURPOSE: Anaplastic thyroid carcinoma (ATC) is one of the most aggressive forms of cancer with no curative therapies available. To date, strategies to target ATC by immunotherapy have not been evaluated. We investigated whether ATC would be a suitable target for natural killer (NK) cell-based immunotherapy.EXPERIMENTAL DESIGN: We first established seven new cell lines from ATC tumors, three from papillary thyroid carcinoma tumors and analyzed them together with eight additional ATC cell lines. Cells were analyzed for sensitivity to lysis by NK cells and their ability to chemoattract and regulate the activity of NK cells. In addition, fresh tumor samples and peripheral blood from six patients with ATC were analyzed for NK cell infiltration and phenotype.RESULTS: We observed that ATC cell lines are sensitive to lysis by ex vivo expanded NK cells and that the lysis was abrogated upon blockade of NKG2D. Sensitivity of thyroid cancer cell lines to NK cell-mediated lysis correlated with surface expression of UL16-binding protein 2 on tumor cells. Moreover, ATC cell lines produced high levels of CXCL10 and stimulated migration of expanded NK cells and ATC tumors were enriched for NK cells expressing the cognate chemokine receptor CXCR3. However, compared with NK cells in peripheral blood, ATC tumor-derived NK cells displayed a suppressed phenotype with a downregulated expression of NKG2D. In vitro, suppression of NK cell-mediated lysis and NKG2D expression by ATC cells was restored upon neutralization of prostaglandin-E2.CONCLUSIONS: ATC cell lines are sensitive to NK cell-mediated lysis via ULBP2/5/6 and chemoattract CXCR3-positive NK cells. Patients with ATC may benefit from NK cell-based immunotherapy.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Pfefferle, AlineKarolinska Institutet,Karolinska Institute
(författare)
-
Ekblad, LarsLund University,Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Huvud- och halscancergruppen,Forskargrupper vid Lunds universitet,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine,Head and Neck Cancer Research Group,Lund University Research Groups,Skåne University Hospital(Swepub:lu)onk-lel
(författare)
-
Yoshimoto, YuyaKarolinska Institute
(författare)
-
Kremer, VeronikaKarolinska Institutet,Karolinska Institute
(författare)
-
Kaminskyy, Vitaliy OKarolinska Institutet,Karolinska Institute
(författare)
-
Juhlin, C ChristoferKarolinska Institutet,Karolinska Institute
(författare)
-
Höög, AndersKarolinska Institutet,Karolinska Institute
(författare)
-
Bodin, IngerKarolinska Institute
(författare)
-
Svjatoha, VitalijsKarolinska Institutet,Karolinska Institute
(författare)
-
Larsson, CatharinaKarolinska Institutet,Karolinska Institute
(författare)
-
Zedenius, JanKarolinska Institutet,Karolinska Institute
(författare)
-
Wennerberg, JohanLund University,Lunds universitet,Öron-, näs- och halssjukdomar, Lund,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Otorhinolaryngology (Lund),Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)onh-jwe
(författare)
-
Lundqvist, AndreasKarolinska Institutet,Karolinska Institute
(författare)
-
Karolinska InstitutetKarolinska Institute
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Clinical Cancer Research20:22, s. 5733-57441078-0432
-
Ingår i:Clinical cancer research : an official journal of the American Association for Cancer Research20:22, s. 5733-57441557-3265
Internetlänk
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Wennerberg, Erik
-
Pfefferle, Aline
-
Ekblad, Lars
-
Yoshimoto, Yuya
-
Kremer, Veronika
-
Kaminskyy, Vital ...
-
visa fler...
-
Juhlin, C Christ ...
-
Höög, Anders
-
Bodin, Inger
-
Svjatoha, Vitali ...
-
Larsson, Cathari ...
-
Zedenius, Jan
-
Wennerberg, Joha ...
-
Lundqvist, Andre ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Cell och molekyl ...
- Artiklar i publikationen
-
Clinical Cancer ...
-
Clinical cancer ...
- Av lärosätet
-
Lunds universitet
-
Karolinska Institutet