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Article leaf apopla...
Article leaf apoplast of field-grown potato analyzed by quantitative proteomics and activity-based protein profiling
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- Abreha, Kibrom Berhe (författare)
- Sveriges lantbruksuniversitet,Swedish University of Agricultural Sciences,Växtskyddsbiologi,Department of Plant Protection Biology
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- Alexandersson, Erik (författare)
- Sveriges lantbruksuniversitet,Swedish University of Agricultural Sciences,Växtskyddsbiologi,Department of Plant Protection Biology
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- Resjö, Svante (författare)
- Sveriges lantbruksuniversitet,Swedish University of Agricultural Sciences,Växtskyddsbiologi,Department of Plant Protection Biology
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- Lankinen, Åsa (författare)
- Sveriges lantbruksuniversitet,Swedish University of Agricultural Sciences,Växtskyddsbiologi,Department of Plant Protection Biology,University of Oxford
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- Sueldo, Daniela (författare)
- University of Oxford
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- Kaschani, Farnusch (författare)
- University of Duisburg-Essen
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- Kaiser, Markus (författare)
- University of Duisburg-Essen
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- van der Hoorn, Renier A.L. (författare)
- University of Oxford
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- Levander, Fredrik (författare)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH,Science for Life Laboratory (SciLifeLab)
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- Andreasson, Erik (författare)
- Sveriges lantbruksuniversitet,Swedish University of Agricultural Sciences,Växtskyddsbiologi,Department of Plant Protection Biology
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(creator_code:org_t)
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- 2021-11-06
- 2021
- Engelska.
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Ingår i: International Journal of Molecular Sciences. - : MDPI AG. - 1661-6596 .- 1422-0067. ; 22:21
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Abstract
Ämnesord
Stäng
- Multiple biotic and abiotic stresses challenge plants growing in agricultural fields. Most molecular studies have aimed to understand plant responses to challenges under controlled conditions. However, studies on field-grown plants are scarce, limiting application of the findings in agricultural conditions. In this study, we investigated the composition of apoplastic proteomes of potato cultivar Bintje grown under field conditions, i.e., two field sites in June–August across two years and fungicide treated and untreated, using quantitative proteomics, as well as its activity using activity-based protein profiling (ABPP). Samples were clustered and some proteins showed significant intensity and activity differences, based on their field site and sampling time (June–August), indicating differential regulation of certain proteins in response to environmental or developmental factors. Peroxidases, class II chitinases, pectinesterases, and osmotins were among the proteins more abundant later in the growing season (July–August) as compared to early in the season (June). We did not detect significant differences between fungicide Shirlan treated and untreated field samples in two growing seasons. Using ABPP, we showed differential activity of serine hydrolases and β-glycosidases under greenhouse and field conditions and across a growing season. Furthermore, the activity of serine hydrolases and β-glycosidases, including proteins related to biotic stress tolerance, decreased as the season progressed. The generated proteomics data would facilitate further studies aiming at understanding mechanisms of molecular plant physiology in agricultural fields and help applying effective strategies to mitigate biotic and abiotic stresses.
Ämnesord
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Jordbruksvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Agricultural Science (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Botanik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Botany (hsv//eng)
Nyckelord
- ABPP
- Apoplast
- Field-omics
- Potato
- Proteomics
- Serine hydrolases
- β-glycosidases
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Abreha, Kibrom B ...
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Alexandersson, E ...
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Resjö, Svante
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Lankinen, Åsa
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Sueldo, Daniela
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Kaschani, Farnus ...
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visa fler...
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Kaiser, Markus
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van der Hoorn, R ...
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Levander, Fredri ...
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Andreasson, Erik
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