Plasma membrane/cytoskeleton interactions in plants

• The way in which the cortical cytoskeleton associates to the plasma membrane (PM) must be elucidated to understand the structural dynamics of many processes in the plant cell. In the present study, isolated cauliflower (Brassica oleracea L.) PM vesicles were used to characterize membrane/cytoskeleton interactions. Both actin and tubulin were shown to copurify with PM vesicles and this copurification was specific to the PM. In order to expose the PM-associated cytoskeleton, Brij 58 was used to form sealed, inside-out vesicles (cytoplasmic side out). Both actin and tubulin colocalized with Brij 58-treated PM vesicles in sucrose-gradient centrifugation, indicating a lack of disruption of the actin/PM and tubulin/PM associations following inside-out vesicle formation. In order to characterize the PM links to actin and tubulin, inside-out PM vesicles were washed in a range of media. Both actin and tubulin co-sedimented with the PM vesicles in the presence of 50 mM DTT, 10 mM CaCl2 and 2 M NaCl (separately). Actin, but not tubulin, was completely released in the presence of 0.6 M KI, and both proteins were released in either 6 M urea or at pH>11.4. The association of actin to the PM was sensitive to extensive dialysis against a low ionic-strength medium. The presence of a pool of a- and b-tubulin with hydrophobic properties, "hydrophobic tubulin", was shown by Triton X-114 fractionation. This hydrophobicity was restricted to PM-associated tubulin and was not seen in soluble tubulin. Hydrophobic tubulin constituted at least 15% of the recovered PM-associated tubulin. Washes in the presence of high concentrations of salt or calcium did not release the hydrophobic tubulin, while high pH (“11.0) did release this fraction. The hydrophobicity of a fraction of the recovered tubulin could reflect a direct or indirect interaction of this tubulin with the PM lipid bilayer or to an integral membrane protein. Hydrophobic tubulin was associated in a salt-sensitive manner to detergent-resistant proteins, likely to be of peripheral/cytoskeletal origin. It is possible that the presence of hydrophobic tubulin in isolated PM reflects the anchoring of the cortical tubulin cytoskeleton to the PM. Further elucidation of the cytoskeleton anchoring mechanism, based on the present study will lead to a better understanding of the structural dynamics of many cellular events.

Ämnesord

NATURVETENSKAP  -- Biologi -- Botanik (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Botany (hsv//eng)

NATURVETENSKAP  -- Biologi -- Cellbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Cell Biology (hsv//eng)

Nyckelord

Triton X-114

hydrophobic tubulin

tubulin

actin

cytoskeleton

Brij 58

inside-out

plasma membrane

plant

cauliflower

Physiological biophysics

Växtfysiologi

Publikations- och innehållstyp

dok (ämneskategori)

vet (ämneskategori)