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  • Waldemarson, SofiaLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH (author)

Protein Expression Changes in Ovarian Cancer during the Transition from Benign to Malignant.

  • Article/chapterEnglish2012

Publisher, publication year, extent ...

  • 2012-04-17
  • American Chemical Society (ACS),2012

Numbers

  • LIBRIS-ID:oai:lup.lub.lu.se:bc5f6a86-fa07-4fe3-be75-94d3465950fd
  • https://lup.lub.lu.se/record/2519865URI
  • https://doi.org/10.1021/pr201258qDOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:124619651URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • Epithelial ovarian carcinoma has in general a poor prognosis since the vast majority of tumors are genomically unstable and clinically highly aggressive. This results in rapid progression of malignancy potential while still asymptomatic and thus in late diagnosis. It is therefore of critical importance to develop methods to diagnose epithelial ovarian carcinoma at its earliest developmental stage, that is, to differentiate between benign tissue and its early malignant transformed counterparts. Here we present a shotgun quantitative proteomic screen of benign and malignant epithelial ovarian tumors using iTRAQ technology with LC-MALDI-TOF/TOF and LC-ESI-QTOF MS/MS. Pathway analysis of the shotgun data pointed to the PI3K/Akt signaling pathway as a significant discriminatory pathway. Selected candidate proteins from the shotgun screen were further confirmed in 51 individual tissue samples of normal, benign, borderline or malignant origin using LC-MRM analysis. The MRM profile demonstrated significant differences between the four groups separating the normal tissue samples from all tumor groups as well as perfectly separating the benign and malignant tumors with a ROC-area of 1. This work demonstrates the utility of using a shotgun approach to filter out a signature of a few proteins only that discriminates between the different sample groups.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Krogh, MortenLund University,Lunds universitet,Beräkningsbiologi och biologisk fysik - Genomgår omorganisation,Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation,Naturvetenskapliga fakulteten,Computational Biology and Biological Physics - Undergoing reorganization,Department of Astronomy and Theoretical Physics - Undergoing reorganization,Faculty of Science(Swepub:lu)thep-mkr (author)
  • Alaiya, Ayodele (author)
  • Kirik, UfukLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)mphy-uki (author)
  • Schedvins, Kjell (author)
  • Auer, GertKarolinska Institutet (author)
  • Hansson, Karin MLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)klke-kh0 (author)
  • Ossola, Reto (author)
  • Aebersold, Ruedi (author)
  • Lee, Hookeun (author)
  • Malmström, JohanLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)medk-jma (author)
  • James, PeterLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)elma-pja (author)
  • Institutionen för immunteknologiInstitutioner vid LTH (creator_code:org_t)

Related titles

  • In:Journal of Proteome Research: American Chemical Society (ACS)11:5, s. 2876-28891535-38931535-3907

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