Identification of the ligand of Pru p 3, a peach LTP

Cubells-Baeza, NuriaTechnical University of Madrid,Centre for Plant Biotechnology and Genomics (author)

Key message: Pru p 3, a peach LTP, is located in pollinated flower styles and secreting downy hairs, transporting a derivative of camptothecin bound to phytosphingosine. Pru p 3 may inhibit a second pollination and may keep away herbivores until seed maturation. Abstract: The allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein. In addition, the described ligand displayed topoisomerase I activity inhibition and self-fluorescence, both recognized as camptothecin properties. During flower development, the highest expression of Pru p 3 was detected in the styles of pollinated flowers, in contrast to its non-expression in unpollinated pistils, where expression decreased after anthesis. During ripening, the expression of Pru p 3 were observed mainly in peel but not in pulp. In this sense, Pru p 3 protein was also localized in trichomes covering the fruit epidermis.

Gómez-Casado, CristinaLund University,Lunds universitet,Slemhinnans immunologi,Forskargrupper vid Lunds universitet,Mucosal Immunology,Lund University Research Groups(Swepub:lu)med-cg_ (author)
Tordesillas, LeticiaIcahn School of Medicine at Mount Sinai (author)
Ramírez-Castillejo, CarmenCentre for Plant Biotechnology and Genomics,Technical University of Madrid (author)
Garrido-Arandia, MaríaCentre for Plant Biotechnology and Genomics,Technical University of Madrid (author)
González-Melendi, PabloTechnical University of Madrid (author)
Herrero, MaríaCSIC - Estacion Experimental de Aula Dei EEAD (author)
Pacios, Luis F.Centre for Plant Biotechnology and Genomics,Technical University of Madrid (author)
Díaz-Perales, AraceliCentre for Plant Biotechnology and Genomics,Technical University of Madrid (author)
Technical University of MadridCentre for Plant Biotechnology and Genomics (creator_code:org_t)

In:Plant Molecular Biology: Springer Science and Business Media LLC94:1-2, s. 33-440167-44121573-5028

By the author/editor: Cubells-Baeza, N ...; Gómez-Casado, Cr ...; Tordesillas, Let ...; Ramírez-Castille ...; Garrido-Arandia, ...; González-Melendi ...; show more...; Herrero, María; Pacios, Luis F.; Díaz-Perales, Ar ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

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