Comparison of (GTG)(5)-oligonucleotide and ribosomal intergenic transcribed spacer (ITS)-PCR for molecular typing of Klebsiella isolates

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Ryberg, AnnaLinköpings universitet,Hälsouniversitetet,Medicinsk mikrobiologi (author)

Comparison of (GTG)(5)-oligonucleotide and ribosomal intergenic transcribed spacer (ITS)-PCR for molecular typing of Klebsiella isolates

Article/chapterEnglish2011

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Elsevier BV,2011

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LIBRIS-ID:oai:lup.lub.lu.se:c0191bc8-d2cf-4ff2-ac07-5915eeb8d0f4
https://lup.lub.lu.se/record/1869174URI
https://doi.org/10.1016/j.mimet.2010.11.019DOI
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-65401URI

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Language:English
Summary in:English

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Subject category:ref swepub-contenttype

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Original Publication: Anna Ryberg, Crister Olsson, Siv Ahrné and Hans-Jürg Monstein, Comparison of (GTG)5-oligonucleotide and ribosomal intergenic transcribed spacer (ITS)-PCR for molecular typing of Klebsiella isolates, 2011, Journal of Microbiological Methods, (84), 2, 183-188. http://dx.doi.org/10.1016/j.mimet.2010.11.019 Copyright: Elsevier Science B.V., Amsterdam. http://www.elsevier.com/
Molecular typing of Klebsiella species has become important for monitoring dissemination of beta-lactamase-producers in hospital environments. The present study was designed to evaluate poly-trinucleotide (GTG)(5)- and rDNA intergenic transcribed spacer (ITS)-PCR fingerprint analysis for typing of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Multiple displacement amplified DNA derived from 19K pneumoniae (some with an ESBL-phenotype). 35 K. oxytoca isolates, five K pneumoniae, two K oxytoca, three Raoultella, and one Enterobacter aerogenes type and reference strains underwent (GTG)(5) and ITS-PCR analysis. Dendrograms were constructed using cosine coefficient and the Neighbour joining method. (GTG)(5) and ITS-PCR analysis revealed that K pneumoniae and K oxytoca isolates. reference and type strains formed distinct cluster groups, and tentative subclusters could be established. We conclude that (GTG)(5) and ITS-PCR analysis combined with automated capillary electrophoresis provides promising tools for molecular typing of Klebsiella isolates. (C) 2010 Elsevier B.V. All rights reserved.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Kirurgi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Surgery hsv//eng
Klebsiella spp.
Molecular typing
(GTG)(5)-PCR
Ribosomal intergenic
transcribed spacer (ITS)-PCR
Fingerprint analysis
Multiple
displacement amplification
Klebsiella spp.; Molecular typing; (GTG)5-PCR; Ribosomal intergenic transcribed spacer (ITS)-PCR; Fingerprint analysis; Multiple displacement amplification
MEDICINE

Added entries (persons, corporate bodies, meetings, titles ...)

Olsson, CristerLund University,Lunds universitet,Kirurgi,Forskargrupper vid Lunds universitet,Surgery,Lund University Research Groups,Malmö University Hospital(Swepub:lu)cr8370ol (author)
Ahrné, SivLund University,Lunds universitet,Avdelningen för livsmedel och läkemedel,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Food and Pharma,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)livt-sah (author)
Monstein, Hans-JürgÖstergötlands Läns Landsting,Linköpings universitet,Klinisk mikrobiologi,Hälsouniversitetet(Swepub:liu)hanmo19 (author)
Linköpings universitetHälsouniversitetet (creator_code:org_t)

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In:Journal of Microbiological Methods: Elsevier BV84:2, s. 183-1881872-83590167-7012

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