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Receptor for alpha1...
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Wester, LLund University
(författare)
Receptor for alpha1-microglobulin on T lymphocytes : inhibition of antigen-induced interleukin-2 production
- Artikel/kapitelEngelska1998
Förlag, utgivningsår, omfång ...
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2002-01-05
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Wiley,1998
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7 s.
Nummerbeteckningar
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LIBRIS-ID:oai:lup.lub.lu.se:c0cce44d-a177-4031-beeb-84674ea8f6bb
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https://lup.lub.lu.se/record/c0cce44d-a177-4031-beeb-84674ea8f6bbURI
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https://doi.org/10.1046/j.1365-3083.1998.00378.xDOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Ämneskategori:ref swepub-contenttype
Anmärkningar
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The human plasma protein alpha1-microglobulin (alpha1m) was found to inhibit the antigen-induced interleukin-2 (IL-2) production of two different mouse T-helper cell hybridomas. Alpha1m isolated from human plasma and recombinant alpha1m isolated from baculovirus-infected insect cell cultures had similar inhibitory effects. Flow cytometric analysis showed a binding of plasma and recombinant alpha1m to the T-cell hybridomas as well as to a human T-cell line. Radiolabelled plasma and recombinant alpha1m bound to the T-cell hybridomas in a saturable manner and the binding could be eliminated by trypsination of the cells. The affinity constants for the cell binding were calculated to be 0.4-1 x 10(5) M(-1) using Scatchard plotting, and the number of binding sites per cell was estimated to be 5 x 10(5)-1 x 10(6). The cell-surface proteins of one of the T-cell hybridomas were radiolabelled, the cells lysed and alpha1m-binding proteins isolated by affinity chromatography. SDS-PAGE and autoradiography analysis of the eluate revealed major bands with Mr-values around 70, 35 and 15 kDa. The results thus suggest that alpha1m binds to a specific receptor on T cells and that the binding leads to inhibition of antigen-stimulated IL-2 production by T-helper cells.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Michaëlsson, ELund University,Lunds universitet,Medicinska fakulteten,Faculty of Medicine(Swepub:lu)smi-emi
(författare)
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Holmdahl, RLund University,Lunds universitet,Immunologi,Forskargrupper vid Lunds universitet,Immunology,Lund University Research Groups(Swepub:lu)infl-rho
(författare)
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Olofsson, TLund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Honungsgruppen,Forskargrupper vid Lunds universitet,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine,Honey group,Lund University Research Groups(Swepub:lu)livs-tol
(författare)
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Åkerström, BLund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Antioxidationsmedicin,Forskargrupper vid Lunds universitet,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Antioxidation medicine,Lund University Research Groups(Swepub:lu)medk-bak
(författare)
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Lund UniversityMedicinska fakulteten
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Scandinavian Journal of Immunology: Wiley48:1, s. 1-70300-94751365-3083
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