Sökning: WFRF:(Wahlgren Mats)
> (2020-2023) >
Direct contact betw...
Direct contact between Plasmodium falciparum and human B-cells in a novel co-culture increases parasite growth and affects B-cell growth
-
- Reddy, Sreenivasulu B. (författare)
- Karolinska Institute
-
- Nagy, Noemi (författare)
- Karolinska Institutet,Karolinska Institute
-
- Rönnberg, Caroline (författare)
- Karolinska Institute,Karolinska University Hospital
-
visa fler...
-
- Chiodi, Francesca (författare)
- Karolinska Institutet,Karolinska Institute
-
- Lugaajju, Allan (författare)
- Karolinska Institute,Makerere University
-
- Heuts, Frank (författare)
- Karolinska Institute
-
- Szekely, Laszlo (författare)
- Karolinska Institutet,Karolinska Institute
-
- Wahlgren, Mats (författare)
- Karolinska Institute
-
- Persson, Kristina E.M. (författare)
- Karolinska Institute,Lund University,Lunds universitet,Malaria och Babesia,Forskargrupper vid Lunds universitet,Malaria and Babesia,Lund University Research Groups,Skåne University Hospital
-
visa färre...
-
(creator_code:org_t)
- 2021-07-05
- 2021
- Engelska.
-
Ingår i: Malaria Journal. - : Springer Science and Business Media LLC. - 1475-2875. ; 20:1
- Relaterad länk:
-
http://dx.doi.org/10... (free)
-
visa fler...
-
https://malariajourn...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Background: Plasmodium falciparum parasites cause malaria and co-exist in humans together with B-cells for long periods of time. Immunity is only achieved after repeated exposure. There has been a lack of methods to mimic the in vivo co-occurrence, where cells and parasites can be grown together for many days, and it has been difficult with long time in vitro studies. Methods and results: A new method for growing P. falciparum in 5% CO2 with a specially formulated culture medium is described. This knowledge was used to establish the co-culture of live P. falciparum together with human B-cells in vitro for 10 days. The presence of B-cells clearly enhanced parasite growth, but less so when Transwell inserts were used (not allowing passage of cells or merozoites), showing that direct contact is advantageous. B-cells also proliferated more in presence of parasites. Symbiotic parasitic growth was verified using CESS cell-line and it showed similar results, indicating that B-cells are indeed the cells responsible for the effect. In malaria endemic areas, people often have increased levels of atypical memory B-cells in the blood, and in this assay it was demonstrated that when parasites were present there was an increase in the proportion of CD19 + CD20 + CD27 − FCRL4 + B-cells, and a contraction of classical memory B-cells. This effect was most clearly seen when direct contact between B-cells and parasites was allowed. Conclusions: These results demonstrate that P. falciparum and B-cells undoubtedly can affect each other when allowed to multiply together, which is valuable information for future vaccine studies.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- B-cell
- Culture
- Human
- Malaria
- Plasmodium falciparum
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas