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- Bakhtiar, ShahrzadLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH (author)
Crystallization and preliminary X-ray analysis of an alkaline serine protease from Nesterenkonia sp. Acta
- Article/chapterEnglish2003
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- 2003
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- LIBRIS-ID:oai:lup.lub.lu.se:c238e3cd-fee7-4423-9957-22abb966575e
- https://lup.lub.lu.se/record/129065URI
- https://doi.org/10.1107/S0907444902020747DOI
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- Language:English
- Summary in:English
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- A novel calcium-independent serine protease from an alkaliphilic bacterium, Nesterenkonia sp. AL20, has been purified and crystallized at 296 K using sodium formate as the main precipitant. This enzyme is optimally active at pH 10, exhibits high stability towards autolytic digestion and its stability is not affected by the presence of EDTA or detergents. The triangular prism-shaped crystals diffracted X-rays to beyond 1.5 Å at a synchrotron beamline, with space group R3 and unit-cell parameters a = b = 92.26, c = 137.88 Å. A complete data set has been collected to 1.39 Å resolution. The asymmetric unit is estimated and confirmed by self-rotation function calculation to contain two molecules, giving a crystal volume per protein mass (VM) of 2.68 Å3 Da-1 and a solvent content of 54%.
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- Vevudova, Jitka (author)
- Hatti-Kaul, RajniLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)biot-rhk (author)
- Su, Xiao-DongLund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)mbfys-xs (author)
- BioteknikCentrum för tillämpade biovetenskaper (creator_code:org_t)
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- In:Acta Crystallographica. Section D: Biological Crystallography59:3, s. 529-5311399-0047
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