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  • White, H (author)

A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR.

  • Article/chapterEnglish2015

Publisher, publication year, extent ...

  • 2014-07-18
  • Springer Science and Business Media LLC,2015

Numbers

  • LIBRIS-ID:oai:lup.lub.lu.se:c3f3c44c-5e55-4655-a579-7edee4d4ca9e
  • https://lup.lub.lu.se/record/4581987URI
  • https://doi.org/10.1038/leu.2014.217DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-247406URI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:130688302URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • Serial quantification of BCR-ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukemia, but there is substantial variation in results reported by different laboratories. To improve comparability, an internationally accepted plasmid certified reference material (CRM) was developed according to ISO Guide 34:2009. Fragments of BCR-ABL1 (e14a2 mRNA fusion), BCR and GUSB transcripts were amplified and cloned into pUC18 to yield plasmid pIRMM0099. Six different linearised plasmid solutions were produced with the following copy number concentrations, assigned by digital PCR, and expanded uncertainties: 1.08±0.13 × 10(6), 1.08±0.11 × 10(5), 1.03±0.10 × 10(4), 1.02±0.09 × 10(3), 1.04±0.10 × 10(2) and 10.0±1.5 copies/μL. The certification of the material for the number of specific DNA fragments per plasmid, copy number concentration of the plasmid solutions and the assessment of inter-unit heterogeneity and stability were performed according to ISO Guide 35:2006. Two suitability studies performed by 63 BCR-ABL1 testing laboratories demonstrated that this set of 6 plasmid CRMs can help to standardise the numbers of measured transcripts of e14a2 BCR-ABL1 and three control genes; ABL1, BCR and GUSB. The set of 6 plasmid CRMs is distributed worldwide by the Institute for Reference Materials and Measurements (Belgium) and its authorised distributors (http://irmm.jrc.ec.europa.eu; CRM code ERM-AD623a-f).Leukemia accepted article preview online, 18 July 2014; doi:10.1038/leu.2014.217.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Deprez, L (author)
  • Corbisier, P (author)
  • Hall, V (author)
  • Lin, F (author)
  • Mazoua, S (author)
  • Trapmann, S (author)
  • Aggerholm, A (author)
  • Andrikovics, H (author)
  • Akiki, S (author)
  • Barbany, GKarolinska Institutet (author)
  • Boeckx, N (author)
  • Bench, A (author)
  • Catherwood, M (author)
  • Jansson, MattiasUppsala universitet,Medicinsk genetik och genomik(Swepub:uu)mattjans (author)
  • Chudleigh, S (author)
  • Clench, T (author)
  • Colomer, D (author)
  • Daraio, F (author)
  • Dulucq, S (author)
  • Farrugia, J (author)
  • Fletcher, L (author)
  • Foroni, L (author)
  • Ganderton, R (author)
  • Gerrard, G (author)
  • Gineikienė, E (author)
  • Hayette, S (author)
  • El Housni, H (author)
  • Izzo, B (author)
  • Jansson, M (author)
  • Johnels, PetraLund University,Lunds universitet,Avdelningen för klinisk genetik,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Genetics,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)kgen-pha (author)
  • Jurcek, T (author)
  • Kairisto, V (author)
  • Kizilors, A (author)
  • Kim, D-W (author)
  • Lange, T (author)
  • Lion, T (author)
  • Polakova, K M (author)
  • Martinelli, G (author)
  • McCarron, S (author)
  • Merle, P A (author)
  • Milner, B (author)
  • Mitterbauer-Hohendanner, G (author)
  • Nagar, M (author)
  • Nickless, G (author)
  • Nomdedéu, J (author)
  • Nymoen, D A (author)
  • Leibundgut, E O (author)
  • Ozbek, U (author)
  • Pajič, T (author)
  • Pfeifer, H (author)
  • Preudhomme, C (author)
  • Raudsepp, K (author)
  • Romeo, G (author)
  • Sacha, T (author)
  • Talmaci, R (author)
  • Touloumenidou, T (author)
  • Van der Velden, V H J (author)
  • Waits, P (author)
  • Wang, L (author)
  • Wilkinson, E (author)
  • Wilson, G (author)
  • Wren, D (author)
  • Zadro, R (author)
  • Ziermann, J (author)
  • Zoi, K (author)
  • Müller, M C (author)
  • Hochhaus, A (author)
  • Schimmel, H (author)
  • Cross, N C P (author)
  • Emons, H (author)
  • Karolinska InstitutetMedicinsk genetik och genomik (creator_code:org_t)

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  • In:Leukemia: Springer Science and Business Media LLC29:2, s. 369-3761476-55510887-6924

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