Liver DNA methylation of FADS2 associates with FADS2 genotypex 06 Biological Sciences 0604 Genetics

Background: Non-alcoholic fatty liver disease has been associated with increased mRNA expression of FADS2 in the liver and estimated activity of delta-6 desaturase in serum, encoded by the FADS2 gene. Since DNA methylation in the FADS1/2/3 gene cluster has been previously linked with genetic variants and desaturase activities, we now aimed to discover factors regulating DNA methylation of the CpG sites annotated to FADS1/2 genes. Methods: DNA methylation levels in the CpG sites annotated to FADS2 and FADS1 were analyzed from liver samples of 95 obese participants of the Kuopio Obesity Surgery Study (34 men and 61 women, age 49.5 ± 7.7 years, BMI 43.0 ± 5.7 kg/m2) using the Infinium HumanMethylation450 BeadChip (Illumina). Associations between DNA methylation levels and estimated delta-6 and delta-5 desaturase enzyme activities, liver histology, hepatic mRNA expression, FADS1/2 genotypes, and erythrocyte folate levels were analyzed. Results: We found a negative correlation between DNA methylation levels of cg06781209 and cg07999042 and hepatic FADS2 mRNA expression (both p < 0.05), and with estimated delta-6 desaturase activity based on both liver and serum fatty acids (all p < 0.05). Interestingly, the methylation level of cg07999042 (p = 0.001) but not of cg06781209 (p = 0.874) was associated with FADS2 variant rs174616. Conclusions: Genetic variants of FADS2 may contribute to the pathogenesis of non-alcoholic fatty liver disease by modifying DNA methylation.

Männistö, VilleKuopio University Hospital,University of Eastern Finland (författare)
De Mello, Vanessa DerenjiUniversity of Eastern Finland (författare)
Vaittinen, MaijaUniversity of Eastern Finland (författare)
Perfilyev, AlexanderLund University,Lunds universitet,Diabetes - epigenetik,Forskargrupper vid Lunds universitet,Diabetes - Epigenetics,Lund University Research Groups(Swepub:lu)med-apy (författare)
Hanhineva, KatiUniversity of Eastern Finland (författare)
Ling, CharlotteLund University,Lunds universitet,Diabetes - epigenetik,Forskargrupper vid Lunds universitet,Diabetes - Epigenetics,Lund University Research Groups(Swepub:lu)endo-cl0 (författare)
Pihlajamäki, JussiKuopio University Hospital,University of Eastern Finland (författare)
University of Eastern FinlandKuopio University Hospital (creator_code:org_t)

Ingår i:Clinical Epigenetics: Springer Science and Business Media LLC11:11868-70751868-7083

Av författaren/redakt...: Walle, Paula; Männistö, Ville; De Mello, Vaness ...; Vaittinen, Maija; Perfilyev, Alexa ...; Hanhineva, Kati; visa fler...; Ling, Charlotte; Pihlajamäki, Jus ...; visa färre...

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