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Affinity tags can reduce merohedral twinning of membrane protein crystals

Backmark, Anna, 1979 (author)
Chalmers University of Technology,Chalmers tekniska högskola
Nyblom, Anna Maria, 1975 (author)
Chalmers University of Technology,Chalmers tekniska högskola
Tornroth-Horsefield, Susanna (author)
Gothenburg University,University of Gothenburg,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
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Kosinska-Eriksson, Urszula, 1976 (author)
Gothenburg University,University of Gothenburg,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
Nordén, Kristina (author)
Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
Agemark, Maria (author)
Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
Kjellbom, Per (author)
Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
Johanson, Urban (author)
Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
Hedfalk, Kristina, 1969 (author)
Gothenburg University,University of Gothenburg,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
Lindkvist- ersson, Karin (author)
University of Gothenburg
Neutze, Richard, 1969 (author)
Gothenburg University,University of Gothenburg,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
Horsefield, Rob, 1977 (author)
Gothenburg University,University of Gothenburg,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
Lindkvist-Petersson, Karin (author)
Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology
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 (creator_code:org_t)
2008
2008
English.
In: Acta Crystallographica. Section D: Biological Crystallography. - 1399-0047 .- 0907-4449. ; D64, s. 1183-1186
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  • Journal article (peer-reviewed)
Abstract Subject headings
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  • This work presents a comparison of the crystal packing of three eukaryotic membrane proteins: human aquaporin 1, human aquaporin 5 and a spinach plasma membrane aquaporin. All were purified from expression constructs both with and without affinity tags. With the exception of tagged aquaporin 1, all constructs yielded crystals. Two significant effects of the affinity tags were observed: crystals containing a tag typically diffracted to lower resolution than those from constructs encoding the protein sequence alone and constructs without a tag frequently produced crystals that suffered from merohedral twinning. Twinning is a challenging crystallographic problem that can seriously hinder solution of the structure. Thus, for integral membrane proteins, the addition of an affinity tag may help to disrupt the approximate symmetry of the protein and thereby reduce or avoid merohedral twinning.

Subject headings

NATURVETENSKAP  -- Biologi -- Strukturbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Structural Biology (hsv//eng)
NATURVETENSKAP  -- Biologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences (hsv//eng)
NATURVETENSKAP  -- Kemi (hsv//swe)
NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)

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