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The Purification an...
The Purification and Characterization of a Cutinase-like Enzyme with Activity on Polyethylene Terephthalate (PET) from a Newly Isolated Bacterium Stenotrophomonas maltophilia PRS8 at a Mesophilic Temperature
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- Din, Salah Ud (författare)
- Lund University,Karadeniz Technical University,Quaid-i-Azam University
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- Satti, Sadia Mehmood (författare)
- Kohsar University Murree
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- Uddin, Salah (författare)
- Quaid-i-Azam University
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- Mankar, Smita V. (författare)
- Lund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Ceylan, Esma (författare)
- Karadeniz Technical University
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- Hasan, Fariha (författare)
- Quaid-i-Azam University
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- Khan, Samiullah (författare)
- Quaid-i-Azam University
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- Badshah, Malik (författare)
- Quaid-i-Azam University
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- Beldüz, Ali Osman (författare)
- Karadeniz Technical University
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- Çanakçi, Sabriye (författare)
- Karadeniz Technical University
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- Zhang, Baozhong (författare)
- Lund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Linares-Pastén, Javier A. (författare)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Shah, Aamer Ali (författare)
- Quaid-i-Azam University
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(creator_code:org_t)
- 2023-03-14
- 2023
- Engelska 22 s.
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Ingår i: Applied Sciences (Switzerland). - : MDPI AG. - 2076-3417. ; 13:6
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- A polyethylene terephthalate (PET)-degrading bacterium identified as Stenotrophomonas maltophilia PRS8 was isolated from the soil of a landfill. The degradation of the PET bottle flakes and the PET prepared as a powder were assessed using live cells, an extracellular medium, or a purified cutinase-like enzyme. These treated polymers were analyzed using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The depolymerization products, identified using HPLC and LC-MS, were terephthalic acid (TPA), mono(2-hydroxyethyl)-TPA (MHET), and bis(2-hydroxyethyl)-TPA (BHET). Several physicochemical factors were optimized for a better cutinase-like enzyme production by using unique single-factor and multi-factor statistical models (the Plackett–Burman design and the central composite design software). The enzyme was purified for homogeneity through column chromatography using Sephadex G-100 resin. The molecular weight of the enzyme was approximately 58 kDa. The specific activity on para nitrophenyl butyrate was estimated at 450.58 U/mg, with a purification of 6.39 times and a yield of 48.64%. The enzyme was stable at various temperatures (30–40 °C) and pH levels (8.0–10.0). The enzyme activity was significantly improved by the surfactants (Triton X-100 and Tween-40), organic solvent (formaldehyde), and metals (NiCl2 and Na2SO4). The extracellular medium containing the cutinase-type enzyme showed a depolymerization yield of the PET powder comparable to that of Idonella skaiensis IsPETase and significantly higher than that of Humicola insolens thermostable HiCut (HiC) cutinase. This study suggests that S. maltophilia PRS8 is able to degrade PET at a mesophilic temperature and could be further explored for the sustainable management of plastic waste.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
Nyckelord
- biodegradation
- PET
- Stenotrophomonas maltophilia
- cutinase
- Plackett–Burman design
- central composite design
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Din, Salah Ud
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Satti, Sadia Meh ...
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Uddin, Salah
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Mankar, Smita V.
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Ceylan, Esma
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Hasan, Fariha
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Khan, Samiullah
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Badshah, Malik
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Beldüz, Ali Osma ...
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Çanakçi, Sabriye
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Zhang, Baozhong
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Linares-Pastén, ...
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Shah, Aamer Ali
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