Impaired airway epithelial cell wound-healing capacity is associated with airway remodelling following RSV infection in severe preschool wheeze

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: (WFRF:(Fleming Louise)) > Impaired airway epi...

Details
MARC

Andersson, Cecilia K.Lund University,Lunds universitet,Respiratorisk cellbiologi,Forskargrupper vid Lunds universitet,Respiratory Cell Biology,Lund University Research Groups,Imperial College London (author)

Impaired airway epithelial cell wound-healing capacity is associated with airway remodelling following RSV infection in severe preschool wheeze

Article/chapterEnglish2020

Publisher, publication year, extent ...

2020-07-15
Wiley,2020
13 s.

Numbers

LIBRIS-ID:oai:lup.lub.lu.se:c994af78-e253-42b2-b253-818eef8116a1
https://lup.lub.lu.se/record/c994af78-e253-42b2-b253-818eef8116a1URI
https://doi.org/10.1111/all.14466DOI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:art swepub-publicationtype
Subject category:ref swepub-contenttype

Notes

Background: Respiratory syncytial virus (RSV) causes exacerbations of asthma and preschool wheeze (PSW). However, the anti-viral and repair responses of the bronchial epithelium in children with severe therapy-resistant asthma (STRA) and PSW are poorly understood. Methods: Children with STRA (age 12 [6-16] years), PSW (age 2 [1-5] years) and non-asthmatic controls (age 7 [2-14] years) underwent bronchoscopy with endobronchial brushings and biopsies. Anti-viral, wound injury responses were quantified in biopsies and primary bronchial epithelial cells (PBECs) in response to RSV, poly(I:C), house dust mite (HDM) or IL-33 using RT-qPCR, Luminex and live cell imaging. Collagen deposition and tissue expression of epithelial growth factor receptor (EGFR), IL-33 and receptor ST2 were investigated in bronchial biopsies. Results: PBECs from STRA and PSW had increased TLR3 gene expression and increased secretion of anti-viral and pro-inflammatory cytokines (IFN-γ, IL-6 and IL-13) in response to RSV compared to controls. Exposure of PBECs to concomitant TLR3 agonist poly(I:C) and HDM resulted in a significant reduction in epithelial cell proliferation in PSW compared to controls. Wound-healing was also impaired in PSW compared to controls at baseline and following IL-33 stimulation. In addition, tissue EGFR expression was significantly reduced in PSW and correlated with collagen deposition in endobronchial biopsies. Conclusions: Despite increased anti-viral responses, preschool children with severe wheeze had impaired airway epithelial proliferative responses following damage. This might be connected to the low expression of EGFR in PSW which may affect epithelial function and contribute to asthma pathogenesis.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Lungmedicin och allergi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Respiratory Medicine and Allergy hsv//eng
epithelium
paediatric asthma
RSV
severe therapy-resistant asthma
wheeze

Added entries (persons, corporate bodies, meetings, titles ...)

Iwasaki, JuaImperial College London (author)
Cook, JamesHarefield Hospital,Imperial College London (author)
Robinson, PollyHarefield Hospital,Imperial College London (author)
Nagakumar, PrasadHarefield Hospital,Imperial College London (author)
Mogren, SofiaLund University,Lunds universitet,Respiratorisk cellbiologi,Forskargrupper vid Lunds universitet,Respiratory Cell Biology,Lund University Research Groups(Swepub:lu)so0187mo (author)
Fleming, LouiseHarefield Hospital (author)
Bush, AndrewHarefield Hospital (author)
Saglani, SejalImperial College London,Harefield Hospital (author)
Lloyd, Clare M.Imperial College London (author)
Respiratorisk cellbiologiForskargrupper vid Lunds universitet (creator_code:org_t)

Related titles

In:Allergy: European Journal of Allergy and Clinical Immunology: Wiley75:12, s. 3195-32070105-4538
In:Allergy: Wiley75:12, s. 3195-32071398-9995

Internet link

Find in a library

Allergy: European Journal of Allergy and Clinical Immunology (Search for host publication in LIBRIS)

To the university's database

Find more in SwePub

By the author/editor: Andersson, Cecil ...; Iwasaki, Jua; Cook, James; Robinson, Polly; Nagakumar, Prasa ...; Mogren, Sofia; show more...; Fleming, Louise; Bush, Andrew; Saglani, Sejal; Lloyd, Clare M.; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Respiratory Medi ...

Articles in the publication: Allergy: Europea ...; Allergy

By the university: Lund University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se