id:"swepub:oai:lup.lub.lu.se:cc3f23bb-77f0-4bff-b0d4-002333776d07"
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- Gisselsson, DavidLund University,Lunds universitet,Cancercellers evolution,Forskargrupper vid Lunds universitet,Pathways of cancer cell evolution,Lund University Research Groups (author)
Clonal evolution through genetic bottlenecks and telomere attrition : Potential threats to in vitro data reproducibility
- Article/chapterEnglish2019
Publisher, publication year, extent ...
- 2018-11-29
- Wiley,2019
Numbers
- LIBRIS-ID:oai:lup.lub.lu.se:cc3f23bb-77f0-4bff-b0d4-002333776d07
- https://lup.lub.lu.se/record/cc3f23bb-77f0-4bff-b0d4-002333776d07URI
- https://doi.org/10.1002/gcc.22685DOI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
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Notes
- Tissue cultures of immortalized human cells, also known as established cell lines, are broadly accessible and cost-efficient tools for biomedical research. We here review potential genetic sources of systematic error in cell line experiments due to clonal evolution in vitro. In particular, the authors highlight alterations in telomere function over prolonged culture and population bottlenecks, respectively, as two commonly overlooked phenomena that can result in significant alterations in cell line genotypes over just one or a few passages in vitro. These alterations may include changes in mutation status of oncogenes and large scale chromosomal imbalances. We introduce a simple list of factors to be avoided in order to reduce the risk of data misinterpretation due to clonal evolution, including unacknowledged in vitro selection pressures, prolonged culture per se, harsh population size reductions, experiments at early phases after establishment, and the employment of cell lines not sufficiently analyzed by high resolution genetic techniques.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
- cancer
- cell lines
- clonal evolution
- genetic bottleneck
- telomeres
Added entries (persons, corporate bodies, meetings, titles ...)
- Lichtenzstejn, DanielUniversity of Manitoba (author)
- Kachko, PolinaBen Gurion University of the Negev (author)
- Karlsson, JennyLund University,Lunds universitet,Cancercellers evolution,Forskargrupper vid Lunds universitet,Pathways of cancer cell evolution,Lund University Research Groups(Swepub:lu)molm-jka (author)
- Manor, EstherBen Gurion University of the Negev (author)
- Mai, SabineUniversity of Manitoba (author)
- Cancercellers evolutionForskargrupper vid Lunds universitet (creator_code:org_t)
Related titles
- In:Genes Chromosomes and Cancer: Wiley58:7, s. 452-4611045-22571098-2264
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