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Loop-Mediated Isoth...
Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications
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- Moore, Keith J M (författare)
- Ateneo de Manila University
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- Cahill, Jeremy (författare)
- Metamer Labs
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- Aidelberg, Guy (författare)
- Center for Research and Interdisciplinarity (CRI)
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- Aronoff, Rachel (författare)
- Center for Research and Interdisciplinarity (CRI)
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- Bektaş, Ali (författare)
- Oakland Genomics Center
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- Bezdan, Daniela (författare)
- University of Tübingen
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- Butler, Daniel J (författare)
- Weill Cornell Medicine
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- Chittur, Sridar V (författare)
- University at Albany, SUNY
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- Codyre, Martin (författare)
- GiantLeap Biotechnology Ltd
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- Federici, Fernan (författare)
- Pontifical Catholic University of Chile
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- Tanner, Nathan A (författare)
- New England Biolabs Inc.
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- Tighe, Scott W (författare)
- University of Vermont
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- True, Randy (författare)
- FloodLAMP Biotechnologies
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- Ware, Sarah B (författare)
- Center for Research and Interdisciplinarity (CRI)
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- Wyllie, Anne L (författare)
- Yale University
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- Afshin, Evan E (författare)
- Weill Cornell Medicine
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- Bendesky, Andres (författare)
- Columbia University
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- Chang, Connie B (författare)
- Montana State University
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- Dela Rosa, Richard (författare)
- Ateneo de Manila University
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- Elhaik, Eran (författare)
- Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Molecular Cell Biology,Department of Biology,Faculty of Science
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- Erickson, David (författare)
- Cornell University
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- Goldsborough, Andrew S (författare)
- RNAssist Ltd
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- Grills, George (författare)
- University of Pennsylvania
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- Hadasch, Kathrin (författare)
- Center for Research and Interdisciplinarity (CRI)
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- Hayden, Andrew (författare)
- University at Albany, SUNY
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- Her, Seong-Young (författare)
- Metamer Labs
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- Karl, Julie A (författare)
- University of Wisconsin-Madison
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- Kim, Chang Hee (författare)
- GoDx
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- Kriegel, Alison J (författare)
- Medical College of Wisconsin
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- Kunstman, Thomas (författare)
- University of Colorado at Boulder
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- Landau, Zeph (författare)
- University of California, Berkeley
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- Land, Kevin (författare)
- University of Pretoria
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- Langhorst, Bradley W (författare)
- New England Biolabs Inc.
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- Lindner, Ariel B (författare)
- Center for Research and Interdisciplinarity (CRI)
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- Mayer, Benjamin E (författare)
- Technical University of Darmstadt
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- McLaughlin, Lee A (författare)
- IC Mobile Lab Ltd
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- McLaughlin, Matthew T (författare)
- University of Wisconsin-Madison
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- Molloy, Jenny (författare)
- University of Cambridge
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- Mozsary, Christopher (författare)
- Weill Cornell Medicine
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- Nadler, Jerry L (författare)
- New York Medical College
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- D'Silva, Melinee (författare)
- New York Medical College
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- Ng, David (författare)
- Columbia University
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- O'Connor, David H (författare)
- University of Wisconsin-Madison
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- Ongerth, Jerry E (författare)
- University of Wollongong
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- Osuolale, Olayinka (författare)
- Elizade University
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- Pinharanda, Ana (författare)
- Columbia University
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- Plenker, Dennis (författare)
- Cold Spring Harbor Laboratory
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- Ranjan, Ravi (författare)
- University of Massachusetts
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- Rosbash, Michael (författare)
- Brandeis University
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- Rotem, Assaf (författare)
- Metamer Labs
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(creator_code:org_t)
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- Association of Biomolecular Resource Facilities, 2021
- 2021
- Engelska.
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Ingår i: Journal of Biomolecular Techniques. - : Association of Biomolecular Resource Facilities. - 1524-0215 .- 1943-4731. ; 32:3, s. 228-275
- Relaterad länk:
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http://dx.doi.org/10...
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https://www.ncbi.nlm...
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https://lup.lub.lu.s...
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https://doi.org/10.7...
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Abstract
Ämnesord
Stäng
- As the second year of the COVID-19 pandemic begins, it remains clear that a massive increase in the ability to test for SARS-CoV-2 infections in a myriad of settings is critical to controlling the pandemic and to preparing for future outbreaks. The current gold standard for molecular diagnostics is the polymerase chain reaction (PCR), but the extraordinary and unmet demand for testing in a variety of environments means that both complementary and supplementary testing solutions are still needed. This review highlights the role that loop-mediated isothermal amplification (LAMP) has had in filling this global testing need, providing a faster and easier means of testing, and what it can do for future applications, pathogens, and the preparation for future outbreaks. This review describes the current state of the art for research of LAMP-based SARS-CoV-2 testing, as well as its implications for other pathogens and testing. The authors represent the global LAMP (gLAMP) Consortium, an international research collective, which has regularly met to share their experiences on LAMP deployment and best practices; sections are devoted to all aspects of LAMP testing, including preanalytic sample processing, target amplification, and amplicon detection, then the hardware and software required for deployment are discussed, and finally, a summary of the current regulatory landscape is provided. Included as well are a series of first-person accounts of LAMP method development and deployment. The final discussion section provides the reader with a distillation of the most validated testing methods and their paths to implementation. This review also aims to provide practical information and insight for a range of audiences: for a research audience, to help accelerate research through sharing of best practices; for an implementation audience, to help get testing up and running quickly; and for a public health, clinical, and policy audience, to help convey the breadth of the effect that LAMP methods have to offer.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- COVID-19/diagnosis
- COVID-19 Nucleic Acid Testing
- Humans
- Molecular Diagnostic Techniques
- Nucleic Acid Amplification Techniques
- Pandemics
- RNA, Viral
- SARS-CoV-2/isolation & purification
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Till lärosätets databas
- Av författaren/redakt...
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Moore, Keith J M
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Cahill, Jeremy
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Aidelberg, Guy
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Aronoff, Rachel
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Bektaş, Ali
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Bezdan, Daniela
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visa fler...
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Butler, Daniel J
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Chittur, Sridar ...
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Codyre, Martin
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Federici, Fernan
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Tanner, Nathan A
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Tighe, Scott W
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True, Randy
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Ware, Sarah B
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Wyllie, Anne L
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Afshin, Evan E
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Bendesky, Andres
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Chang, Connie B
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Dela Rosa, Richa ...
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Elhaik, Eran
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Erickson, David
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Goldsborough, An ...
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Grills, George
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Hadasch, Kathrin
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Hayden, Andrew
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Her, Seong-Young
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Karl, Julie A
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Kim, Chang Hee
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Kriegel, Alison ...
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Kunstman, Thomas
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Landau, Zeph
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Land, Kevin
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Langhorst, Bradl ...
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Lindner, Ariel B
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Mayer, Benjamin ...
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McLaughlin, Lee ...
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McLaughlin, Matt ...
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Molloy, Jenny
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Mozsary, Christo ...
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Nadler, Jerry L
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D'Silva, Melinee
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Ng, David
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O'Connor, David ...
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Ongerth, Jerry E
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Osuolale, Olayin ...
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Pinharanda, Ana
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Plenker, Dennis
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Ranjan, Ravi
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Rosbash, Michael
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Rotem, Assaf
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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och Medicinsk biotek ...
- Artiklar i publikationen
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Journal of Biomo ...
- Av lärosätet
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Lunds universitet