Performance of two immunoassays for the determination of atrazine in sea water samples as compared with on-line solid phase extraction-liquid chromatography-diode array detection

• Two immunoassay formats, magnetic particles-based assay (Atrazine RaPID assay and Atrazine High-Sensitivity RaPID assay) and microtiter plate based assay (Department of Entomology and Environmental Toxicology, University of California in Davis) were evaluated for the determination of atrazine in sea water samples. The results obtained were compared and validated with those obtained by using on-line solid phase extraction followed by liquid chromatography-diode array detection (on-line SPE-LC-DAD). The correlation between both techniques was good when analyzing levels of atrazine ranging from 0.01 to 5 μg/l in samples showing salt concentration values varying from 0 to 35 g/l and pH values from 2 to 10. One of these immunoassays (Atrazine High-Sensitivity RaPID assay) was employed to directly analyze atrazine in real estuarine and coastal water samples. The same samples were analyzed after filtration and C18 Empore disks extraction.

Keyword

Atrazine

Chromatography

Immunoassay

Sea water

Solid-phase extraction

Waters

Publication and Content Type

art (subject category)

ref (subject category)