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Probing skin barrie...
Probing skin barrier recovery on molecular level following acute wounds : An in vivo/ex vivo study on pigs
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- Mojumdar, Enamul Haque (author)
- Malmö universitet,Malmö University,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces
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- Madsen, Lone Bruhn (author)
- Timeline Bioresearch AB
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- Hansson, Henri (author)
- Galenica AB
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- Taavoniku, Ida (author)
- Timeline Bioresearch AB
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- Kristensen, Klaus (author)
- Timeline Bioresearch AB
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- Persson, Christina (author)
- Lund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Morén, Anna Karin (author)
- Galenica AB
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- Mokso, Rajmund (author)
- Lund University,Lunds universitet,Hållfasthetslära,Institutionen för byggvetenskaper,Institutioner vid LTH,Lunds Tekniska Högskola,MAX IV-laboratoriet,Solid Mechanics,Department of Construction Sciences,Departments at LTH,Faculty of Engineering, LTH,MAX IV Laboratory
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- Schmidtchen, Artur (author)
- Lund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Schmidtchen lab,Forskargrupper vid Lunds universitet,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Schmidtchen Lab,Lund University Research Groups,Lund University; University of Copenhagen, Denmark
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- Ruzgas, Tautgirdas (author)
- Malmö universitet,Malmö University,Biofilms Research Center for Biointerfaces,Institutionen för biomedicinsk vetenskap (BMV)
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- Engblom, Johan (author)
- Malmö universitet,Malmö University,Lund University,Lunds universitet,Yrkes- och miljödermatologi,Forskargrupper vid Lunds universitet,Occupational and Environmental Dermatology,Lund University Research Groups,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces
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(creator_code:org_t)
- 2021-03-31
- 2021
- English.
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In: Biomedicines. - : MDPI AG. - 2227-9059. ; 9:4
- Related links:
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https://www.mdpi.com...
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https://doi.org/10.3...
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https://lup.lub.lu.s...
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https://doi.org/10.3...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Proper skin barrier function is paramount for our survival, and, suffering injury, there is an acute need to restore the lost barrier and prevent development of a chronic wound. We hypothesize that rapid wound closure is more important than immediate perfection of the barrier, whereas specific treatment may facilitate perfection. The aim of the current project was therefore to evaluate the quality of restored tissue down to the molecular level. We used Göttingen minipigs with a multi-technique approach correlating wound healing progression in vivo over three weeks, monitored by classical methods (e.g., histology, trans-epidermal water loss (TEWL), pH) and subsequent physicochemical characterization of barrier recovery (i.e., small and wide-angle X-ray diffraction (SWAXD), polarization transfer solid-state NMR (PTssNMR), dynamic vapor sorption (DVS), Fourier transform infrared (FTIR)), providing a unique insight into molecular aspects of healing. We conclude that although acute wounds sealed within two weeks as expected, molecular investigation of stratum corneum (SC) revealed a poorly developed keratin organization and deviations in lipid lamellae formation. A higher lipid fluidity was also observed in regenerated tissue. This may have been due to incomplete lipid conversion during barrier recovery as glycosphingolipids, normally not present in SC, were indicated by infrared FTIR spectroscopy. Evidently, a molecular approach to skin barrier recovery could be a valuable tool in future development of products targeting wound healing.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
Keyword
- Acute wound
- Histology
- In vivo/ex vivo
- Lipid
- PH
- Polarization transfer solid state NMR (PTssNMR)
- Skin barrier
- Small and wide-angle X-ray diffraction (SWAXD)
- Stratum corneum
- Trans-epidermal water loss (TEWL)
- acute wound
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Mojumdar, Enamul ...
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Madsen, Lone Bru ...
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Hansson, Henri
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Taavoniku, Ida
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Kristensen, Klau ...
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Persson, Christi ...
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show more...
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Morén, Anna Kari ...
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Mokso, Rajmund
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Schmidtchen, Art ...
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Ruzgas, Tautgird ...
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Engblom, Johan
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Dermatology and ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Surgery
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Physical Chemist ...
- Articles in the publication
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Biomedicines
- By the university
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Lund University
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Malmö University