Inhibitory effects of PG-H/aggrecan and PG-M/versican on avian neural crest cell migration

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: WFRF:(Bronner Fraser Marianne) > (1996-1999) > Inhibitory effects ...

1 of 1
Previous record
Next record
To hitlist

Details
MARC

Perris, Roberto (author)

Inhibitory effects of PG-H/aggrecan and PG-M/versican on avian neural crest cell migration

Article/chapterEnglish1996

Publisher, publication year, extent ...

1996

Numbers

LIBRIS-ID:oai:lup.lub.lu.se:d76fcdd4-1794-4fb7-8c43-e210259dc1c1
https://lup.lub.lu.se/record/1110702URI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:art swepub-publicationtype
Subject category:ref swepub-contenttype

Notes

Aggrecans and PG-M/versicans represent two newly defined families of hyaluronan-binding proteoglycans for which the function is still poorly understood. Using the avian neural crest as a model system, we have examined the molecular mechanisms entailed in the cell-proteoglycan interaction during embryonic cell motility. Both the primary cartilage aggrecan of the avian embryo (PG-H/aggrecan) and the largest variant of the avian mesenchymal versican (PG-M/versican VO) failed to support neural crest cell adhesion and migration when topographically immobilized onto the substrate. Conversely, solely the PG-H/aggrecan, and similar aggrecans from other species, counteracted the migration-promoting effect of a number of matrix molecules lacking proteoglycan affinity. This inhibitory effect was not reproduced by the isolated glycosaminoglycan chains, the isolated core protein, the reduced and alkylated macromolecule, or the aggrecan in which the G1 hyaluronan-binding domain had been inactivated with hyaluronan fragments or antibodies. Limited depolymerization of the side chains and preincubation of the PG-H/aggrecan with anti-glycosaminoglycan antibodies differentially reduced the inhibitory activity of the proteoglycan on cell motility. The results demonstrate a diverse inhibitory effect of aggrecans and PG-M/versicans on embryonic cell movement and show that the inhibitory action of aggrecans is independent of substrate binding, is dependent on a G1 domain-mediated association of the intact proteoglycan with cell surface-bound hyaluronan, and is differentially mediated by its glycosaminoglycan side chains.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

Perissinotto, Daniela (author)
Pettway, Zoe (author)
Bronner-Fraser, Marianne (author)
Mörgelin, MatthiasLund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)medk-mmn (author)
Kimata, Koji (author)
InfektionsmedicinSektion III (creator_code:org_t)

Related titles

In:FASEB Journal10:2, s. 293-3011530-6860

Internet link

Find in a library

FASEB Journal (Search for host publication in LIBRIS)

To the university's database

1 of 1
Previous record
Next record
To hitlist

Find more in SwePub

By the author/editor: Perris, Roberto; Perissinotto, Da ...; Pettway, Zoe; Bronner-Fraser, ...; Mörgelin, Matthi ...; Kimata, Koji

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

Articles in the publication: FASEB Journal

By the university: Lund University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se