SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:lup.lub.lu.se:d8cb376b-159f-405e-9c62-51e1d52dc26f"
 

Search: onr:"swepub:oai:lup.lub.lu.se:d8cb376b-159f-405e-9c62-51e1d52dc26f" > Spatio-temporal act...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
  • Rodhe, JohannaKarolinska Institutet,Karolinska Institute (author)

Spatio-temporal activation of caspase-8 in myeloid cells upon ischemic stroke

  • Article/chapterEnglish2016

Publisher, publication year, extent ...

  • 2016-08-26
  • Springer Science and Business Media LLC,2016

Numbers

  • LIBRIS-ID:oai:lup.lub.lu.se:d8cb376b-159f-405e-9c62-51e1d52dc26f
  • https://lup.lub.lu.se/record/d8cb376b-159f-405e-9c62-51e1d52dc26fURI
  • https://doi.org/10.1186/s40478-016-0365-9DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:136449337URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:art swepub-publicationtype
  • Subject category:ref swepub-contenttype

Notes

  • Ischemic stroke (caused by thrombosis, embolism or vasoconstriction) lead to the recruitment and activation of immune cells including resident microglia and infiltrating peripheral macrophages, which contribute to an inflammatory response involved in regulation of the neuronal damage. We showed earlier that upon pro-inflammatory stimuli, the orderly activation of caspase-8 and caspase-3/7 regulates microglia activation through a protein kinase C-δ dependent pathway. Here, we present in vivo evidence for the activation of caspase-8 and caspase-3 in microglia/macrophages in post-mortem tissue from human ischemic stroke subjects. Indeed, CD68-positive microglia/macrophages in the ischemic peri-infarct area exhibited significant expression of the cleaved and active form of caspase-8 and caspase-3. The temporal and spatial activation of caspase-8 was further investigated in a permanent middle cerebral artery occlusion mouse model of ischemic stroke. Increasing levels of active caspase-8 was found in Iba1-positive cells over time in the peri-infarct area, at 6, 24 and 48 h after artery occlusion. Analysis of post-mortem brain tissue from human subject who suffered two stroke events, referred as recent and old stroke, revealed that expression of cleaved caspase-8 and -3 in CD68-positive cells could only be found in the recent stroke area. Analysis of cleaved caspase-8 and -3 expressions in a panel of human stroke cases arranged upon days-after stroke and age-matched controls suggested that the expression of these caspases correlated with the time of onset of stroke. Collectively, these data illustrate the temporal and spatial activation of caspase-8 and -3 in microglia/macrophages occurring upon ischemic stroke and suggest that the expression of these caspases could be used in neuropathological diagnostic work.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Burguillos, Miguel A. (author)
  • de Pablos, Rocio M.University of Seville (author)
  • Kavanagh, Edel (author)
  • Persson, AnnetteLund University,Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)pat-ape (author)
  • Englund, ElisabetLund University,Lunds universitet,CNS Genterapi,Forskargrupper vid Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,CNS Gene Therapy,Lund University Research Groups,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine(Swepub:lu)pat-een (author)
  • Deierborg, TomasLund University,Lunds universitet,CNS Genterapi,Forskargrupper vid Lunds universitet,Neuroinflammation,CNS Gene Therapy,Lund University Research Groups(Swepub:lu)expb-tol (author)
  • Venero, Jose L.University of Seville (author)
  • Joseph, BertrandKarolinska Institutet (author)
  • Karolinska InstitutetKarolinska Institute (creator_code:org_t)

Related titles

  • In:Acta Neuropathologica Communications: Springer Science and Business Media LLC4, s. 1-112051-5960

Internet link

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view